Clarkson, Christopher T and Deeks, Emma A and Samarista, Ralph and Mamayusupova, Hulkar and Zhurkin, Victor B and Teif, Vladimir B (2019) CTCF-dependent chromatin boundaries formed by asymmetric nucleosome arrays with decreased linker length. Nucleic Acids Research, 47 (21). pp. 11181-11196. DOI https://doi.org/10.1093/nar/gkz908
Clarkson, Christopher T and Deeks, Emma A and Samarista, Ralph and Mamayusupova, Hulkar and Zhurkin, Victor B and Teif, Vladimir B (2019) CTCF-dependent chromatin boundaries formed by asymmetric nucleosome arrays with decreased linker length. Nucleic Acids Research, 47 (21). pp. 11181-11196. DOI https://doi.org/10.1093/nar/gkz908
Clarkson, Christopher T and Deeks, Emma A and Samarista, Ralph and Mamayusupova, Hulkar and Zhurkin, Victor B and Teif, Vladimir B (2019) CTCF-dependent chromatin boundaries formed by asymmetric nucleosome arrays with decreased linker length. Nucleic Acids Research, 47 (21). pp. 11181-11196. DOI https://doi.org/10.1093/nar/gkz908
Abstract
The CCCTC-binding factor (CTCF) organises the genome in 3D through DNA loops and in 1D by setting boundaries isolating different chromatin states, but these processes are not well understood. Here we investigate chromatin boundaries in mouse embryonic stem cells, defined by the regions with decreased Nucleosome Repeat Length (NRL) for ~20 nucleosomes near CTCF sites, affecting up to 10% of the genome. We found that the nucleosome-depleted region (NDR) near CTCF is asymmetrically located >40 nucleotides 5’-upstream from the centre of CTCF motif. The strength of CTCF binding to DNA and the presence of cohesin is correlated with the decrease of NRL near CTCF, and anti-correlated with the level of asymmetry of the nucleosome array. Individual chromatin remodellers have different contributions, with Snf2h having the strongest effect on the NRL decrease near CTCF and Chd4 playing a major role in the symmetry breaking. Upon differentiation, a subset of preserved, common CTCF sites maintains asymmetric nucleosome pattern and small NRL. The sites which lost CTCF upon differentiation are characterised by nucleosome rearrangement 3’-downstream, with unchanged NDR 5’-upstream of CTCF motifs. Boundaries of topologically associated chromatin domains frequently contain several inward-oriented CTCF motifs whose effects, described above, add up synergistically.
Item Type: | Article |
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Uncontrolled Keywords: | nucleosome; chromatin; CTCF; gene regulation |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 23 Oct 2019 14:28 |
Last Modified: | 07 Aug 2024 20:46 |
URI: | http://repository.essex.ac.uk/id/eprint/25701 |
Available files
Filename: gkz908.pdf
Licence: Creative Commons: Attribution 3.0