Sideri, Theodora and Rallis, Charalampos and Bitton, Danny A and Lages, Bruno M and Suo, Fang and Rodríguez-López, María and Du, Li-Lin and Bähler, Jürg (2015) Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence. G3: Genes, Genomes, Genetics, 5 (1). pp. 145-155. DOI https://doi.org/10.1534/g3.114.014415
Sideri, Theodora and Rallis, Charalampos and Bitton, Danny A and Lages, Bruno M and Suo, Fang and Rodríguez-López, María and Du, Li-Lin and Bähler, Jürg (2015) Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence. G3: Genes, Genomes, Genetics, 5 (1). pp. 145-155. DOI https://doi.org/10.1534/g3.114.014415
Sideri, Theodora and Rallis, Charalampos and Bitton, Danny A and Lages, Bruno M and Suo, Fang and Rodríguez-López, María and Du, Li-Lin and Bähler, Jürg (2015) Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence. G3: Genes, Genomes, Genetics, 5 (1). pp. 145-155. DOI https://doi.org/10.1534/g3.114.014415
Abstract
Genetic factors underlying aging are remarkably conserved from yeast to human. The fission yeast Schizosaccharomyces pombe is an emerging genetic model to analyze cellular aging. Chronological lifespan (CLS) has been studied in stationary-phase yeast cells depleted for glucose, which only survive for a few days. Here, we analyzed CLS in quiescent S. pombe cells deprived of nitrogen, which arrest in a differentiated, G0-like state and survive for more than 2 months. We applied parallel mutant phenotyping by barcode sequencing (Bar-seq) to assay pooled haploid deletion mutants as they aged together during long-term quiescence. As expected, mutants with defects in autophagy or quiescence were under-represented or not detected. Lifespan scores could be calculated for 1199 mutants. We focus the discussion on the 48 most long-lived mutants, including both known aging genes in other model systems and genes not previously implicated in aging. Genes encoding membrane proteins were particularly prominent as pro-aging factors. We independently verified the extended CLS in individual assays for 30 selected mutants, showing the efficacy of the screen. We also applied Bar-seq to profile all pooled deletion mutants for proliferation under a standard growth condition. Unlike for stationary-phase cells, no inverse correlation between growth and CLS of quiescent cells was evident. These screens provide a rich resource for further studies, and they suggest that the quiescence model can provide unique, complementary insights into cellular aging.
Item Type: | Article |
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Uncontrolled Keywords: | Schizosaccharomyces; DNA, Fungal; Mutation; DNA Barcoding, Taxonomic |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 18 Jun 2021 14:55 |
Last Modified: | 07 Aug 2024 19:22 |
URI: | http://repository.essex.ac.uk/id/eprint/26714 |
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Filename: Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.pdf
Licence: Creative Commons: Attribution 3.0