Grydaki, Nikoletta and Colbeck, Ian and Mendes, Luis and Eleftheriadis, Konstantinos and Whitby, Corinne (2021) Bioaerosols in the Athens Metro: Metagenetic insights into the PM₁₀ microbiome in a naturally ventilated subway station. Environment International, 146. p. 106186. DOI https://doi.org/10.1016/j.envint.2020.106186
Grydaki, Nikoletta and Colbeck, Ian and Mendes, Luis and Eleftheriadis, Konstantinos and Whitby, Corinne (2021) Bioaerosols in the Athens Metro: Metagenetic insights into the PM₁₀ microbiome in a naturally ventilated subway station. Environment International, 146. p. 106186. DOI https://doi.org/10.1016/j.envint.2020.106186
Grydaki, Nikoletta and Colbeck, Ian and Mendes, Luis and Eleftheriadis, Konstantinos and Whitby, Corinne (2021) Bioaerosols in the Athens Metro: Metagenetic insights into the PM₁₀ microbiome in a naturally ventilated subway station. Environment International, 146. p. 106186. DOI https://doi.org/10.1016/j.envint.2020.106186
Abstract
To date, few studies have examined the aerosol microbial content in Metro transportation systems. Here we characterised the aerosol microbial abundance, diversity and composition in the Athens underground railway system. PM10 filter samples were collected from the naturally ventilated Athens Metro Line 3 station “Nomismatokopio”. Quantitative PCR of the 16S rRNA gene and high throughput amplicon sequencing of the 16S rRNA gene and internal transcribed spacer (ITS) region was performed on DNA extracted from PM10 samples. Results showed that, despite the bacterial abundance (mean = 2.82 × 105 16S rRNA genes/m3 of air) being, on average, higher during day-time and weekdays, compared to night-time and weekends, respectively, the differences were not statistically significant. The average PM10 mass concentration on the platform was 107 μg/m3. However, there was no significant correlation between 16S rRNA gene abundance and overall PM10 levels. The Athens Metro air microbiome was mostly dominated by bacterial and fungal taxa of environmental origin (e.g. Paracoccus, Sphingomonas, Cladosporium, Mycosphaerella, Antrodia) with a lower contribution of human commensal bacteria (e.g. Corynebacterium, Staphylococcus). This study highlights the importance of both outdoor air and commuters as sources in shaping aerosol microbial communities. To our knowledge, this is the first study to characterise the mycobiome diversity in the air of a Metro environment based on amplicon sequencing of the ITS region. In conclusion, this study presents the first microbial characterisation of PM10 in the Athens Metro, contributing to the growing body of microbiome exploration within urban transit networks. Moreover, this study shows the vulnerability of public transport to airborne disease transmission.
Item Type: | Article |
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Uncontrolled Keywords: | Metro; Bioaerosols; PM10; Bacteria; Fungi; High throughput sequencing |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 25 Nov 2020 10:23 |
Last Modified: | 30 Oct 2024 16:23 |
URI: | http://repository.essex.ac.uk/id/eprint/28970 |
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Licence: Creative Commons: Attribution 3.0