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Genome-wide screens in yeast models towards understanding chronological lifespan regulation

Legon, Luc and Rallis, Charalampos (2021) 'Genome-wide screens in yeast models towards understanding chronological lifespan regulation.' Briefings in Functional Genomics. ISSN 1473-9550

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Abstract

Cellular models such as yeasts are a driving force in biogerontology studies. Their simpler genome, short lifespans and vast genetic and genomics resources make them ideal to characterise pro-ageing and anti-ageing genes and signalling pathways. Over the last three decades, yeasts have contributed to the understanding of fundamental aspects of lifespan regulation including the roles of nutrient response, global protein translation rates and quality, DNA damage, oxidative stress, mitochondrial function and dysfunction as well as autophagy. In this short review, we focus on approaches used for competitive and non-competitive cell-based screens using the budding yeast Saccharomyces cerevisiae, and the fission yeast Schizosaccharomyces pombe, for deciphering the molecular mechanisms underlying chronological ageing. Automation accompanied with appropriate computational tools allowed manipulation of hundreds of thousands of colonies, generation, processing and analysis of genome-wide lifespan data. Together with barcoding and modern mutagenesis technologies, these approaches have allowed to take decisive steps towards a global, comprehensive view of cellular ageing.

Item Type: Article
Uncontrolled Keywords: ageing, budding yeast, fission yeast, chronological, phenomics, TOR
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Elements
Date Deposited: 23 Mar 2021 16:11
Last Modified: 23 Mar 2021 17:15
URI: http://repository.essex.ac.uk/id/eprint/30070

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