Uttarotai, Toungporn and Sutheeworapong, Sawannee and Crombie, Andrew T and Murrell, J Colin and Mhuantong, Wuttichai and Noirungsee, Nuttapol and Wangkarn, Sunanta and Bovonsombut, Sakunnee and McGenity, Terry J and Chitov, Thararat (2022) Genome Characterisation of an Isoprene-Degrading Alcaligenes sp. Isolated from a Tropical Restored Forest. Biology, 11 (4). p. 519. DOI https://doi.org/10.3390/biology11040519
Uttarotai, Toungporn and Sutheeworapong, Sawannee and Crombie, Andrew T and Murrell, J Colin and Mhuantong, Wuttichai and Noirungsee, Nuttapol and Wangkarn, Sunanta and Bovonsombut, Sakunnee and McGenity, Terry J and Chitov, Thararat (2022) Genome Characterisation of an Isoprene-Degrading Alcaligenes sp. Isolated from a Tropical Restored Forest. Biology, 11 (4). p. 519. DOI https://doi.org/10.3390/biology11040519
Uttarotai, Toungporn and Sutheeworapong, Sawannee and Crombie, Andrew T and Murrell, J Colin and Mhuantong, Wuttichai and Noirungsee, Nuttapol and Wangkarn, Sunanta and Bovonsombut, Sakunnee and McGenity, Terry J and Chitov, Thararat (2022) Genome Characterisation of an Isoprene-Degrading Alcaligenes sp. Isolated from a Tropical Restored Forest. Biology, 11 (4). p. 519. DOI https://doi.org/10.3390/biology11040519
Abstract
Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. Strain 13f was isolated from soil beneath wild Himalayan cherry trees in a tropical restored forest. Based on phylogenomic analysis and an Average Nucleotide Identity score of >95%, it most probably belongs to the species <i>Alcaligenes faecalis</i>. Isoprene degradation by <i>Alcaligenes</i> sp. strain 13f was measured by using gas chromatography. When isoprene was supplied as the sole carbon and energy source at the concentration of 7.2 × 10<sup>5</sup> ppbv and 7.2 × 10<sup>6</sup> ppbv, 32.6% and 19.6% of isoprene was consumed after 18 days, respectively. Genome analysis of <i>Alcaligenes</i> sp. strain 13f revealed that the genes that are typically found as part of the isoprene monooxygenase gene cluster in other isoprene-degrading bacteria were absent. This discovery suggests that there may be alternative pathways for isoprene metabolism.
Item Type: | Article |
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Uncontrolled Keywords: | isoprene; climate-active gas; isoprene degradation; genome; Alcaligenes |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 14 Sep 2022 09:01 |
Last Modified: | 07 Aug 2024 19:35 |
URI: | http://repository.essex.ac.uk/id/eprint/33476 |
Available files
Filename: biology-11-00519.pdf
Licence: Creative Commons: Attribution 3.0