Chiduza, George N and Johnson, Rachel M and Wright, Gareth SA and Antonyuk, Svetlana V and Muench, Stephen P and Hasnain, S Samar (2019) LAT1 (SLC7A5) and CD98hc (SLC3A2) complex dynamics revealed by single-particle cryo-EM. Acta Crystallographica Section D Structural Biology, 75 (7). pp. 660-669. DOI https://doi.org/10.1107/s2059798319009094
Chiduza, George N and Johnson, Rachel M and Wright, Gareth SA and Antonyuk, Svetlana V and Muench, Stephen P and Hasnain, S Samar (2019) LAT1 (SLC7A5) and CD98hc (SLC3A2) complex dynamics revealed by single-particle cryo-EM. Acta Crystallographica Section D Structural Biology, 75 (7). pp. 660-669. DOI https://doi.org/10.1107/s2059798319009094
Chiduza, George N and Johnson, Rachel M and Wright, Gareth SA and Antonyuk, Svetlana V and Muench, Stephen P and Hasnain, S Samar (2019) LAT1 (SLC7A5) and CD98hc (SLC3A2) complex dynamics revealed by single-particle cryo-EM. Acta Crystallographica Section D Structural Biology, 75 (7). pp. 660-669. DOI https://doi.org/10.1107/s2059798319009094
Abstract
Solute carriers are a large class of transporters that play key roles in normal and disease physiology. Among the solute carriers, heteromeric amino-acid transporters (HATs) are unique in their quaternary structure. LAT1–CD98hc, a HAT, transports essential amino acids and drugs across the blood–brain barrier and into cancer cells. It is therefore an important target both biologically and therapeutically. During the course of this work, cryo-EM structures of LAT1–CD98hc in the inward-facing conformation and in either the substrate-bound or apo states were reported to 3.3–3.5 Å resolution [Yan<jats:italic>et al.</jats:italic>(2019),<jats:italic>Nature (London)</jats:italic>,<jats:bold>568</jats:bold>, 127–130]. Here, these structures are analyzed together with our lower resolution cryo-EM structure, and multibody 3D auto-refinement against single-particle cryo-EM data was used to characterize the dynamics of the interaction of CD98hc and LAT1. It is shown that the CD98hc ectodomain and the LAT1 extracellular surface share no substantial interface. This allows the CD98hc ectodomain to have a high degree of movement within the extracellular space. The functional implications of these aspects are discussed together with the structure determination.
Item Type: | Article |
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Uncontrolled Keywords: | transporters; cryo-EM; amino-acid transporters; solute carriers; LAT1; CD98hc; SLC7A5; SLC3A2 |
Divisions: | Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 28 Aug 2025 18:41 |
Last Modified: | 28 Aug 2025 18:41 |
URI: | http://repository.essex.ac.uk/id/eprint/34758 |
Available files
Filename: LAT1 (SLC7A5) and CD98hc (SLC3A2) complex dynamics revealed by single-particle cryo-EM.pdf
Licence: Creative Commons: Attribution 4.0