Puig-Barbe, Aleix and Dettmann, Svenja and Nirello, Vinícius Dias and Moor, Helen and Azami, Sina and Edgar, Bruce A and Varga-Weisz, Patrick and Korzelius, Jerome and de Navascués, Joaquín (2025) A bHLH interaction code controls bipotential differentiation and self-renewal in the Drosophila gut. Cell Reports, 44 (3). p. 115398. DOI https://doi.org/10.1016/j.celrep.2025.115398
Puig-Barbe, Aleix and Dettmann, Svenja and Nirello, Vinícius Dias and Moor, Helen and Azami, Sina and Edgar, Bruce A and Varga-Weisz, Patrick and Korzelius, Jerome and de Navascués, Joaquín (2025) A bHLH interaction code controls bipotential differentiation and self-renewal in the Drosophila gut. Cell Reports, 44 (3). p. 115398. DOI https://doi.org/10.1016/j.celrep.2025.115398
Puig-Barbe, Aleix and Dettmann, Svenja and Nirello, Vinícius Dias and Moor, Helen and Azami, Sina and Edgar, Bruce A and Varga-Weisz, Patrick and Korzelius, Jerome and de Navascués, Joaquín (2025) A bHLH interaction code controls bipotential differentiation and self-renewal in the Drosophila gut. Cell Reports, 44 (3). p. 115398. DOI https://doi.org/10.1016/j.celrep.2025.115398
Abstract
Multipotent adult stem cells balance self-renewal with differentiation into various cell types. How this balance is regulated at the transcriptional level is poorly understood. Here, we show that a network of basic helix-loop-helix (bHLH) transcription factors controls both stemness and bipotential differentiation in the Drosophila adult intestine. We find that homodimers of Daughterless (Da), a homolog of mammalian E proteins, maintain self-renewal of intestinal stem cells (ISCs), antagonizing the enteroendocrine fate promoted by heterodimers of Da and Scute (Sc; homolog of ASCL). The HLH factor Extramacrochaetae (Emc; homologous to Id proteins) promotes absorptive differentiation by titrating Da and Sc. Emc prevents the committed absorptive progenitor from dedifferentiating, underscoring the plasticity of these cells. Switching physical interaction partners in this way enables the active maintenance of stemness while priming stem cells for differentiation along two alternative fates. Such regulatory logic is likely operative in other bipotent stem cell systems.
Item Type: | Article |
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Uncontrolled Keywords: | Animals; Basic Helix-Loop-Helix Transcription Factors; Cell Differentiation; Cell Self Renewal; DNA-Binding Proteins; Drosophila melanogaster; Drosophila Proteins; Intestines; Repressor Proteins; Stem Cells; Transcription Factors |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 01 Aug 2025 12:12 |
Last Modified: | 01 Aug 2025 12:12 |
URI: | http://repository.essex.ac.uk/id/eprint/41362 |
Available files
Filename: mmc4.pdf
Licence: Creative Commons: Attribution 4.0