Green, Benjamin C and Suggett, David J and Hills, Alan and Steinke, Michael (2012) Optimisation of a fast DMS sensor (FDS) for real time quantification of dimethyl sulfide production by algae. Biogeochemistry, 110 (1-3). pp. 163-172. DOI https://doi.org/10.1007/s10533-011-9678-8
Green, Benjamin C and Suggett, David J and Hills, Alan and Steinke, Michael (2012) Optimisation of a fast DMS sensor (FDS) for real time quantification of dimethyl sulfide production by algae. Biogeochemistry, 110 (1-3). pp. 163-172. DOI https://doi.org/10.1007/s10533-011-9678-8
Green, Benjamin C and Suggett, David J and Hills, Alan and Steinke, Michael (2012) Optimisation of a fast DMS sensor (FDS) for real time quantification of dimethyl sulfide production by algae. Biogeochemistry, 110 (1-3). pp. 163-172. DOI https://doi.org/10.1007/s10533-011-9678-8
Abstract
Production of dimethyl sulfide (DMS) from marine samples is often quantified using gas chromatography techniques. Typically, these are labour intensive and have a slow sample turnover rate. Here we demonstrate the use of a portable fast DMS sensor (FDS) that utilises the chemiluminescent reaction of DMS and ozone to measure DMS production in aqueous samples, with a maximum frequency of 10 Hz. We have developed a protocol for quantifying DMS production that removes potential signal interference from other biogenic trace gases such as isoprene (2-methyl-1,3-butadiene) and hydrogen sulfide. The detection limit was 0.89 pM (0.02 ppbv) when using a DMS standard gas mixture. The lowest DMS production rates quantified with the FDS and verified using conventional gas chromatography with flame photometric detection (GC-FPD) were around 0.01 nmol min−1. There was a strong correlation in DMS production when comparing the FDS and GC-FPD techniques with a range of marine samples (e.g., r 2 = 0.94 for Emiliania huxleyi). However, the combined dataset showed the FDS measured 22% higher DMS production than the GC-FPD, with the differences in rates likely due to interfering gases, for example hydrogen sulfide and isoprene. This possible overestimation of DMS production is smaller than the two-fold difference in DMS production between day and night samples from a culture of E. huxleyi. The response time of the instrument to changes in DMS production is method dependent (e.g., geometry of incubation vessel, bubble size) and was approximately 4 min under our conditions when using a culture of E. huxleyi (800 ml) with aeration at 100 ml min−1. We suggest the FDS can reduce sample handling, is suitable for short- and long-term measurements of DMS production in algal cultures, and will widen the range of DMS research in marine environments.
Item Type: | Article |
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Additional Information: | Published proceedings: Biogeochemistry |
Uncontrolled Keywords: | Dimethyl sulfide (DMS); DMS production; Method; Fast DMS sensor; Chemiluminescence detector |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 15 Feb 2013 17:08 |
Last Modified: | 16 May 2024 16:13 |
URI: | http://repository.essex.ac.uk/id/eprint/5653 |
Available files
Filename: Green et al FAST DMS SENSOR Biogeochem 110 2012.pdf