Wolfe, Gordon V and Steinke, Michael (1996) Grazing‐activated production of dimethyl sulfide (DMS) by two clones of <i>Emiliania huxleyi</i>. Limnology and Oceanography, 41 (6). pp. 1151-1160. DOI https://doi.org/10.4319/lo.1996.41.6.1151
Wolfe, Gordon V and Steinke, Michael (1996) Grazing‐activated production of dimethyl sulfide (DMS) by two clones of <i>Emiliania huxleyi</i>. Limnology and Oceanography, 41 (6). pp. 1151-1160. DOI https://doi.org/10.4319/lo.1996.41.6.1151
Wolfe, Gordon V and Steinke, Michael (1996) Grazing‐activated production of dimethyl sulfide (DMS) by two clones of <i>Emiliania huxleyi</i>. Limnology and Oceanography, 41 (6). pp. 1151-1160. DOI https://doi.org/10.4319/lo.1996.41.6.1151
Abstract
<jats:p><jats:italic>Emiliania huxleyi</jats:italic> clones CCMP 370 and CCMP 373 produced similar amounts of dimethylsulfoniopropionate (DMSP) during axenic exponential growth, averaging 109 mM internal DMSP. Both clones had detectable DMSP lyase activity, as measured by production of dimethyl sulfide (DMS) during in vitro assays of crude cell preparations, but activities and conditions differed considerably between clones. Clone 373 had high activity; clone 370 had low activity and required chloride. For both strains, enzyme activity per cell was constant during exponential growth, but little DMS was produced by healthy cells. Rather, DMS production was activated when cells were subjected to physical or chemical stresses that caused cell lysis. We propose that DMSP lyase and DMSP are segregated within these cells and reaction only under conditions that result in cell stress or damage. Such activation occurs during microzooplankton grazing. When these clones were grazed by the dinoflagellate <jats:italic>Oxyrrhis marina,</jats:italic> DMS was produced; ungrazed cells, as well as those exposed to grazer exudates and associated bacteria, generated no DMS. Grazing of clone 373 produced much more DMS than grazing of clone 370, consistent with their relative in vitro DMSP lyase activities. DMS was only generated when cells were actually being grazed, indicating that ingested cells were responsible for the DMS formation. We suggest that even low levels of grazing can greatly accelerate DMS production.</jats:p>
Item Type: | Article |
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Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 25 Mar 2013 10:09 |
Last Modified: | 16 May 2024 17:43 |
URI: | http://repository.essex.ac.uk/id/eprint/5672 |
Available files
Filename: Wolfe and Steinke L&O 1996.pdf