Steinke, M and Daniel, C and Kirst, GO (1996) DMSP lyase in marine macro- and microalgae: interspecific differences in cleavage activity. In: Biological and Environmental Chemistry of DMSP and Related Sulfonium Compounds. Plenum, New York, pp. 317-324. ISBN 9780306453069. Official URL: https://doi.org/10.1007/978-1-4613-0377-0_27
Steinke, M and Daniel, C and Kirst, GO (1996) DMSP lyase in marine macro- and microalgae: interspecific differences in cleavage activity. In: Biological and Environmental Chemistry of DMSP and Related Sulfonium Compounds. Plenum, New York, pp. 317-324. ISBN 9780306453069. Official URL: https://doi.org/10.1007/978-1-4613-0377-0_27
Steinke, M and Daniel, C and Kirst, GO (1996) DMSP lyase in marine macro- and microalgae: interspecific differences in cleavage activity. In: Biological and Environmental Chemistry of DMSP and Related Sulfonium Compounds. Plenum, New York, pp. 317-324. ISBN 9780306453069. Official URL: https://doi.org/10.1007/978-1-4613-0377-0_27
Abstract
The enzymatic cleavage of dimethylsulfoniopropionate (DMSP) to dimethylsulfide (DMS) was investigated in twenty-one strains of marine macro- and microalgae, representing seven algal classes. The enzymes involved in this cleavage are DMSP lyases, producing DMS from DMSP. All algal strains tested were able to synthesize and accumulate various levels of intracellular DMSP but only twelve strains showed DMSP lyase activity. It was possible to identify subgroups of strong and weak DMS producers. The first subgroup included three Enteromorpha species (E. clathrata, E. intestinalis, E. compressa) and Phaeocystis sp. with specific activities in crude cell extracts ranging from 7 to over 100 nmol DMS min-1 (mg cell protein)-1. The second subgroup was composed of a sub-antarctic strain of Acrosiphonia arcta, Polysiphonia lanosa, two strains of Emiliania huxleyi, Acrosiphonia sonderi, Ulva lactuca and Enteromorpha bulbosa. In this subgroup activity ranged from 0.01 to 0.2 nmol DMS min-1 (mg cell protein)-1. No DMSP lyase was detectable in a sub-arctic strain of Acrosiphonia arcta, Acrosiphonia sonderi, Monostroma arcticum, Prasiola crispa, Polysiphonia urceolata, Ascoseira mirabilis, Laminaria saccharina and Tetraselmis subcordiformis. Non-optimal assay conditions and bacterial contamination may have affected rates in some samples, but the results suggest the widespread presence of DMSP lyase among algal taxa, and also raises the possibility that closely-related species may have quite different lyase activities or function.
Item Type: | Book Section |
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Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 25 Mar 2013 10:03 |
Last Modified: | 16 May 2024 18:54 |
URI: | http://repository.essex.ac.uk/id/eprint/5674 |
Available files
Filename: Steinke et al 1st DMSP 1996.pdf