Driever, Steven M and Fryer, Michael J and Mullineaux, Philip M and Baker, Neil R (2009) Imaging of Reactive Oxygen Species In vivo. Methods in molecular biology (Clifton, N.J.), 479. pp. 109-116. DOI https://doi.org/10.1007/978-1-59745-289-2_7
Driever, Steven M and Fryer, Michael J and Mullineaux, Philip M and Baker, Neil R (2009) Imaging of Reactive Oxygen Species In vivo. Methods in molecular biology (Clifton, N.J.), 479. pp. 109-116. DOI https://doi.org/10.1007/978-1-59745-289-2_7
Driever, Steven M and Fryer, Michael J and Mullineaux, Philip M and Baker, Neil R (2009) Imaging of Reactive Oxygen Species In vivo. Methods in molecular biology (Clifton, N.J.), 479. pp. 109-116. DOI https://doi.org/10.1007/978-1-59745-289-2_7
Abstract
Reactive oxygen species (ROS) are involved in many signalling pathways and numerous stress responses in plants. Consequently, it is important to be able to identify and localize ROS in vivo to evaluate their roles in signalling. A number of probes that have a high affinity for specific ROS and that are effectively taken up by cells and tissues are commercially available. Applications to intact leaves of singlet oxygen sensor green (SOSG), nitroblue tetrazolium (NBT), di-amino benzidine (DAB) and Amplex Red to detect singlet oxygen, superoxide and hydrogen peroxide are described. Imaging of the probes in the cells and tissues of leaves allows sites of ROS production to be identified.
Item Type: | Article |
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Uncontrolled Keywords: | Arabidopsis; Plant Leaves; Hydrogen Peroxide; Superoxides; Singlet Oxygen; Reactive Oxygen Species |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 15 Sep 2011 12:33 |
Last Modified: | 16 May 2024 16:22 |
URI: | http://repository.essex.ac.uk/id/eprint/723 |