Waring, Jen and Klenell, Markus and Bechtold, Ulrike and Underwood, Graham JC and Baker, Neil R (2010) LIGHT‐INDUCED RESPONSES OF OXYGEN PHOTOREDUCTION, REACTIVE OXYGEN SPECIES PRODUCTION AND SCAVENGING IN TWO DIATOM SPECIES<sup>1</sup>. Journal of Phycology, 46 (6). pp. 1206-1217. DOI https://doi.org/10.1111/j.1529-8817.2010.00919.x
Waring, Jen and Klenell, Markus and Bechtold, Ulrike and Underwood, Graham JC and Baker, Neil R (2010) LIGHT‐INDUCED RESPONSES OF OXYGEN PHOTOREDUCTION, REACTIVE OXYGEN SPECIES PRODUCTION AND SCAVENGING IN TWO DIATOM SPECIES<sup>1</sup>. Journal of Phycology, 46 (6). pp. 1206-1217. DOI https://doi.org/10.1111/j.1529-8817.2010.00919.x
Waring, Jen and Klenell, Markus and Bechtold, Ulrike and Underwood, Graham JC and Baker, Neil R (2010) LIGHT‐INDUCED RESPONSES OF OXYGEN PHOTOREDUCTION, REACTIVE OXYGEN SPECIES PRODUCTION AND SCAVENGING IN TWO DIATOM SPECIES<sup>1</sup>. Journal of Phycology, 46 (6). pp. 1206-1217. DOI https://doi.org/10.1111/j.1529-8817.2010.00919.x
Abstract
<jats:p>Diatoms are frequently exposed to high light (HL) levels, which can result in photoinhibition and damage to PSII. Many microalgae can photoreduce oxygen using the Mehler reaction driven by PSI, which could protect PSII. The ability of <jats:italic>Nitzschia epithemioides</jats:italic> Grunow and <jats:italic>Thalassiosira pseudonana</jats:italic> Hasle et Heimdal grown at 50 and 300 μmol photons · m<jats:sup>−2</jats:sup> · s<jats:sup>−1</jats:sup> to photoreduce oxygen was examined by mass spectrometric measurements of <jats:sup>18</jats:sup>O<jats:sub>2</jats:sub>. Both species exhibited significant rates of oxygen photoreduction at saturating light levels, with cells grown in HL exhibiting higher rates. HL‐grown <jats:italic>T. pseudonana</jats:italic> had maximum rates of oxygen photoreduction five times greater than <jats:italic>N. epithemoides</jats:italic>, with 49% of electrons transported through PSII being used to reduce oxygen. Exposure to excess light (1,000 μmol photons · m<jats:sup>−2</jats:sup> · s<jats:sup>−1</jats:sup>) produced similar decreases in the operating quantum efficiency of PSII (<jats:italic>F</jats:italic><jats:sub>q</jats:sub>′/<jats:italic>F</jats:italic><jats:sub>m</jats:sub>′) of low light (LL)‐ and HL‐grown <jats:italic>N. epithemoides</jats:italic>, whereas HL‐grown <jats:italic>T. pseudonana</jats:italic> exhibited much smaller decreases in <jats:italic>F</jats:italic><jats:sub>q</jats:sub>′/<jats:italic>F</jats:italic><jats:sub>m</jats:sub>′ than LL‐grown cells. HL‐grown <jats:italic>T. pseudonana</jats:italic> and <jats:italic>N. epithemioides</jats:italic> exhibited greater superoxide and hydrogen peroxide production, higher activities (in <jats:italic>T. pseudonana</jats:italic>) of superoxide dismutase (SOD) and ascorbate peroxidase (APX), and increased expression of three SOD‐ and one APX‐encoding genes after 60 min of excess light compared to LL‐grown cells. These responses provide a mechanism that contributes to the photoprotection of PSII against photodamage.</jats:p>
Item Type: | Article |
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Uncontrolled Keywords: | ascorbate peroxidase; chl fluorescence; Mehler reaction; microphytobenthos; photosynthesis; phytoplankton; PSII photochemistry; superoxide dismutase |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of Faculty of Science and Health > Computer Science and Electronic Engineering, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 06 Oct 2011 14:54 |
Last Modified: | 16 May 2024 16:17 |
URI: | http://repository.essex.ac.uk/id/eprint/763 |