Bischin, Cristina and Deac, Florina and Silaghi-Dumitrescu, Radu and Worrall, Jonathan AR and Rajagopal, Badri S and Damian, Grigore and Cooper, Chris E (2011) Ascorbate peroxidase activity of cytochrome<i>c</i>. Free Radical Research, 45 (4). pp. 439-444. DOI https://doi.org/10.3109/10715762.2010.540575
Bischin, Cristina and Deac, Florina and Silaghi-Dumitrescu, Radu and Worrall, Jonathan AR and Rajagopal, Badri S and Damian, Grigore and Cooper, Chris E (2011) Ascorbate peroxidase activity of cytochrome<i>c</i>. Free Radical Research, 45 (4). pp. 439-444. DOI https://doi.org/10.3109/10715762.2010.540575
Bischin, Cristina and Deac, Florina and Silaghi-Dumitrescu, Radu and Worrall, Jonathan AR and Rajagopal, Badri S and Damian, Grigore and Cooper, Chris E (2011) Ascorbate peroxidase activity of cytochrome<i>c</i>. Free Radical Research, 45 (4). pp. 439-444. DOI https://doi.org/10.3109/10715762.2010.540575
Abstract
The peroxidase-type reactivity of cytochrome c is proposed to play a role in free radical production and/or apoptosis. This study describes cytochrome c catalysis of peroxide consumption by ascorbate. Under conditions where the sixth coordination position at the cytochrome c heme iron becomes more accessible for exogenous ligands (by carboxymethylation, cardiolipin addition or by partial denaturation with guanidinium hydrochloride) this peroxidase activity is enhanced. A reaction intermediate is detected by stopped-flow UV-vis spectroscopy upon reaction of guanidine-treated cytochrome c with peroxide, which resembles the spectrum of globin Compound II species and is thus proposed to be a ferryl species. The ability of physiological levels of ascorbate (10μM) to interact with this species may have implications for mechanisms of cell signalling or damage that are based on cytochrome c/peroxide interactions. © 2011 Informa UK, Ltd.
Item Type: | Article |
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Uncontrolled Keywords: | Cytochrome c; peroxide; ascorbate |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 23 Sep 2011 08:53 |
Last Modified: | 07 Aug 2024 19:41 |
URI: | http://repository.essex.ac.uk/id/eprint/796 |