Mohr, Andrea and Zwacka, Ralf M (2007) <i>In situ</i> trapping of initiator caspases reveals intermediate surprises. Cell Biology International, 31 (5). pp. 526-530. DOI https://doi.org/10.1016/j.cellbi.2006.11.016
Mohr, Andrea and Zwacka, Ralf M (2007) <i>In situ</i> trapping of initiator caspases reveals intermediate surprises. Cell Biology International, 31 (5). pp. 526-530. DOI https://doi.org/10.1016/j.cellbi.2006.11.016
Mohr, Andrea and Zwacka, Ralf M (2007) <i>In situ</i> trapping of initiator caspases reveals intermediate surprises. Cell Biology International, 31 (5). pp. 526-530. DOI https://doi.org/10.1016/j.cellbi.2006.11.016
Abstract
<jats:title>Abstract</jats:title><jats:p>A novel method to identify initiator caspases is an <jats:italic>in situ</jats:italic> trapping approach using a cell‐permeable biotinylated caspase inhibitor valine‐alanine‐aspartate‐fluoromethyl ketone (bVAD) that binds covalently and irreversibly to the active cysteine site of caspases. This inhibits apoptosis and should allow precipitation of initiator caspases in their uncleaved forms. However, in our experiments TRAIL and FasL‐induced apoptosis and bVAD labelling did not result in streptavidin precipitation of the procaspase‐8 forms, but led to the pull‐down of the intermediate and to a lesser extent fully cleaved forms (p41/43 and p18). These findings are contrary to other reports and are of relevance to apoptosis research as they challenge the general concept of the bVAD approach that procaspases are being trapped. We show that (partially) processed forms of initiator caspases rather than procaspases might be precipitated with this method.</jats:p>
Item Type: | Article |
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Uncontrolled Keywords: | initiator caspases; apoptosis; procaspases |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 29 Jun 2017 09:56 |
Last Modified: | 07 Aug 2024 20:07 |
URI: | http://repository.essex.ac.uk/id/eprint/8325 |