Griffiths‐Jones, Sam and Hui, Jerome HL and Marco, Antonio and Ronshaugen, Matthew (2011) MicroRNA evolution by arm switching. EMBO reports, 12 (2). pp. 172-177. DOI https://doi.org/10.1038/embor.2010.191
Griffiths‐Jones, Sam and Hui, Jerome HL and Marco, Antonio and Ronshaugen, Matthew (2011) MicroRNA evolution by arm switching. EMBO reports, 12 (2). pp. 172-177. DOI https://doi.org/10.1038/embor.2010.191
Griffiths‐Jones, Sam and Hui, Jerome HL and Marco, Antonio and Ronshaugen, Matthew (2011) MicroRNA evolution by arm switching. EMBO reports, 12 (2). pp. 172-177. DOI https://doi.org/10.1038/embor.2010.191
Abstract
<jats:p>MicroRNAs (miRNAs) modulate transcript stability and translation. Functional mature miRNAs are processed from one or both arms of the hairpin precursor. The miR‐100/10 family has undergone three independent evolutionary events that have switched the arm from which the functional miRNA is processed. The dominant miR‐10 sequences in the insects <jats:italic>Drosophila melanogaster</jats:italic> and <jats:italic>Tribolium castaneum</jats:italic> are processed from opposite arms. However, the duplex produced by Dicer cleavage has an identical sequence in fly and beetle. Expression of the <jats:italic>Tribolium</jats:italic> miR‐10 sequence in <jats:italic>Drosophila</jats:italic> S2 cells recapitulates the native beetle pattern. Thus, arm usage is encoded in the primary miRNA sequence, but outside the mature miRNA duplex. We show that the predicted messenger RNA targets and inferred function of sequences from opposite arms differ significantly. Arm switching is likely to be general, and provides a fundamental mechanism to evolve the function of a miRNA locus and target gene network.</jats:p>
Item Type: | Article |
---|---|
Uncontrolled Keywords: | microRNA; evolution; Hox; Drosophila; Tribolium |
Subjects: | Q Science > Q Science (General) |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 23 Mar 2015 14:32 |
Last Modified: | 30 Oct 2024 20:35 |
URI: | http://repository.essex.ac.uk/id/eprint/8496 |
Available files
Filename: 2011_EMBOReports_ArmSwitching.pdf
Licence: Creative Commons: Attribution-Noncommercial-No Derivative Works 3.0