Metodieva, Gergana and Greenwood, Christina and Alldridge, Louise and Sauven, Paul and Metodiev, Metodi (2009) A peptide-centric approach to breast cancer biomarker discovery utilizing label-free multiple reaction monitoring mass spectrometry. PROTEOMICS - Clinical Applications, 3 (1). pp. 78-82. DOI https://doi.org/10.1002/prca.200800072
Metodieva, Gergana and Greenwood, Christina and Alldridge, Louise and Sauven, Paul and Metodiev, Metodi (2009) A peptide-centric approach to breast cancer biomarker discovery utilizing label-free multiple reaction monitoring mass spectrometry. PROTEOMICS - Clinical Applications, 3 (1). pp. 78-82. DOI https://doi.org/10.1002/prca.200800072
Metodieva, Gergana and Greenwood, Christina and Alldridge, Louise and Sauven, Paul and Metodiev, Metodi (2009) A peptide-centric approach to breast cancer biomarker discovery utilizing label-free multiple reaction monitoring mass spectrometry. PROTEOMICS - Clinical Applications, 3 (1). pp. 78-82. DOI https://doi.org/10.1002/prca.200800072
Abstract
We report an approach for multiplex analysis of cancer biomarkers based on the measurement of diagnostic peptides in whole tissue protein digests. Label-free quantitation with MS3 multiple reaction monitoring (MRM) was developed to afford accurate analysis of prospective marker peptides in a panel of breast tumors. This approach provides an economical and robust alternative to stable isotope-based methods. It is equally applicable to the analysis of samples derived from tissue biopsy, aspirate, or plasma and can be easily translated to clinic. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Biomarkers; Breast cancer; Mass spectrometry; Nanoelectrospray; Peptides |
| Subjects: | R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) |
| Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
| SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
| Depositing User: | Unnamed user with email elements@essex.ac.uk |
| Date Deposited: | 06 Oct 2011 13:21 |
| Last Modified: | 23 Sep 2022 18:30 |
| URI: | http://repository.essex.ac.uk/id/eprint/869 |