Metodieva, Gergana and Greenwood, Christina and Alldridge, Louise and Sauven, Paul and Metodiev, Metodi (2009) A peptide‐centric approach to breast cancer biomarker discovery utilizing label‐free multiple reaction monitoring mass spectrometry. PROTEOMICS – Clinical Applications, 3 (1). pp. 78-82. DOI https://doi.org/10.1002/prca.200800072
Metodieva, Gergana and Greenwood, Christina and Alldridge, Louise and Sauven, Paul and Metodiev, Metodi (2009) A peptide‐centric approach to breast cancer biomarker discovery utilizing label‐free multiple reaction monitoring mass spectrometry. PROTEOMICS – Clinical Applications, 3 (1). pp. 78-82. DOI https://doi.org/10.1002/prca.200800072
Metodieva, Gergana and Greenwood, Christina and Alldridge, Louise and Sauven, Paul and Metodiev, Metodi (2009) A peptide‐centric approach to breast cancer biomarker discovery utilizing label‐free multiple reaction monitoring mass spectrometry. PROTEOMICS – Clinical Applications, 3 (1). pp. 78-82. DOI https://doi.org/10.1002/prca.200800072
Abstract
<jats:title>Abstract</jats:title><jats:p>We report an approach for multiplex analysis of cancer biomarkers based on the measurement of diagnostic peptides in whole tissue protein digests. Label‐free quantitation with MS3 multiple reaction monitoring (MRM) was developed to afford accurate analysis of prospective marker peptides in a panel of breast tumors. This approach provides an economical and robust alternative to stable isotope‐based methods. It is equally applicable to the analysis of samples derived from tissue biopsy, aspirate, or plasma and can be easily translated to clinic.</jats:p>
Item Type: | Article |
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Uncontrolled Keywords: | Biomarkers; Breast cancer; Mass spectrometry; Nanoelectrospray; Peptides |
Subjects: | R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 06 Oct 2011 13:21 |
Last Modified: | 04 Dec 2024 06:22 |
URI: | http://repository.essex.ac.uk/id/eprint/869 |