Research Repository

Transformation methods for halophilic archaebacteria

Cline, SW and Lam, WL and Charlebois, RL and Schalkwyk, LC and Doolittle, WF (1989) 'Transformation methods for halophilic archaebacteria.' Canadian Journal of Microbiology, 35 (1). 148 - 152. ISSN 0008-4166

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We present a practical description of polyethylene glycol mediated spheroplast transformation of Halobacterium halobium and Halobacterium volcanii. This method has been applied to phage DNA transfection, plasmid DNA transformation, and transformation with linear fragments of high molecular weight genomic DNA. Efficient spheroplast regeneration allows uncomplicated recovery of transformed progeny. Transformations can be performed equally well using fresh or frozen cell preparations. These methods should find application in molecular cloning, genetic fine mapping, and strain construction.

Item Type: Article
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 03 Aug 2017 11:31
Last Modified: 23 Aug 2019 10:15

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