Kõks, S and Velthut, A and Sarapik, A and Altmäe, S and Reinmaa, E and Schalkwyk, LC and Fernandes, C and Lad, HV and Soomets, U and Jaakma, U and Salumets, A (2009) 'The differential transcriptome and ontology profiles of floating and cumulus granulosa cells in stimulated human antral follicles.' Molecular Human Reproduction, 16 (4). 229 - 240. ISSN 1360-9947

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Abstract

Communication between various ovarian cell types is a prerequisite for folliculogenesis and ovulation. In antral follicles gran-ulosa cells divide into two distinct populations of mural and cumulus granulosa cells (CGC), enveloping the antrum and surrounding the oocyte, respectively. Both cell types, with the mural compartment in excess, contribute to the floating granulosa cell (FGC) population in the follicular fluid. The aim of this study was to compare the transcriptomes of FGC and CGC in stimulated antral follicles obtained from 19 women undergoing IVF-ICSI procedure. FGC were obtained from follicular fluid during the follicle puncture procedure and CGC were acquired after oocyte denudation for micromanipulation. Gene expression analysis was conducted using the genome-wide Affymetrix transcriptome array. The expression profile of the two granulosa cell populations varied significantly. Out of 28 869 analysed transcripts 4480 were differentially expressed (q-value > 10 -4 ) and 489 showed ≥2-fold difference in the expression level with 222 genes up-regulated in FGC and 267 in CGC. The transcriptome of FGC showed higher expression of genes involved in immune response, hematological system function and organismal injury, although CGC had genes involved in protein degradation and nervous system function up-regulated. Cell-to-cell signalling and interaction pathways were noted in both cell populations. Furthermore, numerous novel transcripts that have not been previously described in follicular physiology were identified. In conclusion, our results provide a solid basis for future studies in follicular biology that will help to identify molecular markers for oocyte and embryo viability in IVF. © The Author 2009. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org.

Item Type:	Article
Subjects:	Q Science > QH Natural history > QH426 Genetics
Divisions:	Faculty of Science and Health > Biological Sciences, School of
Depositing User:	Jim Jamieson
Date Deposited:	14 Nov 2014 17:04
Last Modified:	17 Aug 2017 17:46
URI:	http://repository.essex.ac.uk/id/eprint/11052

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