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Quantitative traits for the tail suspension test: automation, optimization, and BXD RI mapping

Lad, Heena V and Liu, Lin and Payá-Cano, José L and Fernandes, Cathy and Schalkwyk, Leonard C (2007) 'Quantitative traits for the tail suspension test: automation, optimization, and BXD RI mapping.' Mammalian Genome, 18 (6-7). pp. 482-491. ISSN 0938-8990

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Immobility in the tail suspension test (TST) is considered a model of despair in a stressful situation, and acute treatment with antidepressants reduces immobility. Inbred strains of mouse exhibit widely differing baseline levels of immobility in the TST and several quantitative trait loci (QTLs) have been nominated. The labor of manual scoring and various scoring criteria make obtaining robust data and comparisons across different laboratories problematic. Several studies have validated strain gauge and video analysis methods by comparison with manual scoring. We set out to find objective criteria for automated scoring parameters that maximize the biological information obtained, using a video tracking system on tapes of tail suspension tests of 24 lines of the BXD recombinant inbred panel and the progenitor strains C57BL/6J and DBA/2J. The maximum genetic effect size is captured using the highest time resolution and a low mobility threshold. Dissecting the trait further by comparing genetic association of multiple measures reveals good evidence for loci involved in immobility on chromosomes 4 and 15. These are best seen when using a high threshold for immobility, despite the overall better heritability at the lower threshold. A second trial of the test has greater duration of immobility and a completely different genetic profile. Frequency of mobility is also an independent phenotype, with a distal chromosome 1 locus. © 2007 Springer Science+Business Media, LLC.

Item Type: Article
Uncontrolled Keywords: Animals; Mice, Inbred Strains; Mice; Mice, Mutant Strains; Chromosome Mapping; Crosses, Genetic; Immobilization; Hindlimb Suspension; Genotype; Lod Score; Phenotype; Quantitative Trait Loci; Research Design; Automation; Male
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 17 Dec 2014 19:23
Last Modified: 15 Jan 2022 00:41

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