Research Repository

DNA methylation at the Igf2/H19 imprinting control region is associated with cerebellum mass in outbred mice

Pidsley, R and Fernandes, C and Viana, J and Paya-Cano, JL and Liu, L and Smith, RG and Schalkwyk, LC and Mill, J (2012) 'DNA methylation at the Igf2/H19 imprinting control region is associated with cerebellum mass in outbred mice.' Molecular Brain, 5. ISSN 1756-6606

[img]
Preview
Text
1756-6606-5-42.pdf - Published Version
Available under License Creative Commons Attribution.

Download (477kB) | Preview

Abstract

Background: Insulin-like growth factor 2 (Igf2) is a paternally expressed imprinted gene regulating fetal growth, playing an integral role in the development of many tissues including the brain. The parent-of-origin specific expression of Igf2 is largely controlled by allele-specific DNA methylation at CTCF-binding sites in the imprinting control region (ICR), located immediately upstream of the neighboring H19 gene. Previously we reported evidence of a negative correlation between DNA methylation in this region and cerebellum weight in humans. Results: We quantified cerebellar DNA methylation across all four CTCF binding sites spanning the murine Igf2/H19 ICR in an outbred population of Heterogeneous Stock (HS) mice (n = 48). We observe that DNA methylation at the second and third CTCF binding sites in the Igf2/H19 ICR shows a negative relationship with cerebellar mass, reflecting the association observed in human post-mortem cerebellum tissue. Conclusions: Given the important role of the cerebellum in motor control and cognition, and the link between structural cerebellar abnormalities and neuropsychiatric phenotypes, the identification of epigenetic factors associated with cerebellum growth and development may provide important insights about the etiology of psychiatric disorders.

Item Type: Article
Uncontrolled Keywords: Igf2; H19; Epigenetics; DNA methylation; Cerebellum; Brain; Mouse; Genotype; Genomic imprinting
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Science and Health > Biological Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 11 Nov 2014 13:16
Last Modified: 23 May 2018 11:15
URI: http://repository.essex.ac.uk/id/eprint/11087

Actions (login required)

View Item View Item