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Mood-stabilizers differentially affect housekeeping gene expression in human cells

Powell, Timothy R and Powell-Smith, Georgia and Haddley, Kate and Mcguffin, Peter and Quinn, John and Schalkwyk, Leonard C and Farmer, Anne E and D'Souza, Ursula M (2014) 'Mood-stabilizers differentially affect housekeeping gene expression in human cells.' International Journal of Methods in Psychiatric Research, 23 (2). pp. 279-288. ISSN 1049-8931

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Recent studies have revealed that antidepressants affect the expression of constitutively expressed "housekeeping genes" commonly used as normalizing reference genes in quantitative polymerase chain reaction (qPCR) experiments. There has yet to be an investigation however on the effects of mood-stabilizers on housekeeping gene stability. The current study utilized lymphoblastoid cell lines (LCLs) derived from patients with mood disorders to investigate the effects of a range of doses of lithium (0, 1, 2 and 5mM) and sodium valproate (0, 0.06, 0.03 and 0.6mM) on the stability of 12 housekeeping genes. RNA was extracted from LCLs and qPCR was used to generate cycle threshold (Ct) values which were input into RefFinder analyses. The study revealed drug-specific effects on housekeeping gene stability. The most stable housekeeping genes in LCLs treated: acutely with sodium valproate were ACTB and RPL13A; acutely with lithium were GAPDH and ATP5B; chronically with lithium were ATP5B and CYC1. The stability of GAPDH and B2M were particularly affected by duration of lithium treatment. The study adds to a growing literature that the selection of appropriate housekeeping genes is important for the accurate normalization of target gene expression in experiments investigating the molecular effects of mood disorder pharmacotherapies. © 2014 John Wiley & Sons, Ltd.

Item Type: Article
Uncontrolled Keywords: reference genes; lithium; sodium valproate; gene expression normalization
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 23 Oct 2014 12:12
Last Modified: 15 Jan 2022 00:42

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