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Effect of chronic valproic acid treatment on hepatic gene expression profile in wfs1 knockout mouse

Punapart, M and Eltermaa, M and Oflijan, J and Sütt, S and Must, A and Kõks, S and Schalkwyk, LC and Fernandes, C and Vasar, E and Soomets, U and Terasmaa, A (2014) 'Effect of chronic valproic acid treatment on hepatic gene expression profile in wfs1 knockout mouse.' PPAR Research, 2014. ISSN 1687-4757

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Abstract

Valproic acid (VPA) is a widely used anticonvulsant and mood-stabilizing drug whose use is often associated with drug-induced weight gain. Treatment with VPA has been shown to upregulate Wfs1 expression in vitro. Aim of the present study was to compare the effect of chronic VPA treatment in wild type (WT) and Wfs1 knockout (KO) mice on hepatic gene expression profile. Wild type, Wfs1 heterozygous, and homozygous mice were treated with VPA for three months (300 mg/kg i.p. daily) and gene expression profiles in liver were evaluated using Affymetrix Mouse GeneChip 1.0 ST array. We identified 42 genes affected by Wfs1 genotype, 10 genes regulated by VPA treatment, and 9 genes whose regulation by VPA was dependent on genotype. Among the genes that were regulated differentially by VPA depending on genoty pe was peroxisome proliferator- activated receptor delta (Ppard), whose expression was upregulated in response to VPA treatment in WT, but not in Wfs1 KO mice. Thus, regulation of Ppard by VPA is dependent on Wfs1 genotype. © 2014 Marite Punapart et al.

Item Type: Article
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Science and Health > Biological Sciences, School of
Depositing User: Jim Jamieson
Date Deposited: 23 Oct 2014 12:33
Last Modified: 20 Feb 2018 11:15
URI: http://repository.essex.ac.uk/id/eprint/11097

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