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NMR studies of BPTI aggregation by using paramagnetic relaxation reagents

Bernini, A and Spiga, O and Ciutti, A and Venditti, V and Prischi, F and Governatori, M and Bracci, L and Lelli, B and Pileri, S and Botta, M and Barge, A and Laschi, F and Niccolai, N (2006) 'NMR studies of BPTI aggregation by using paramagnetic relaxation reagents.' Biochimica et Biophysica Acta - Proteins and Proteomics, 1764 (5). 856 - 862. ISSN 1570-9639

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Abstract

Paramagnetic probes, whose approach to proteins can be monitored by nuclear magnetic resonance (NMR) studies, have been found of primary relevance for investigating protein surfaces accessibility. Here, paramagnetic probes are also suggested for a systematic investigation on protein aggregation. Bovin pancreatic trypsin inhibitor (BPTI) was used as a model system for aggregation by analyzing its interaction with TEMPOL and Gd(III)DTPA-BMA. Some of the measured paramagnetic relaxation rates of BPTI protons exhibited a reverse dependence on protein concentration, which can be attributed to the formation of transient BPTI aggregates. © 2006 Elsevier B.V. All rights reserved.

Item Type: Article
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Science and Health > Life Sciences, School of
Depositing User: Filippo Prischi
Date Deposited: 04 Feb 2016 12:57
Last Modified: 14 Oct 2019 17:17
URI: http://repository.essex.ac.uk/id/eprint/14842

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