Bernini, Andrea and Spiga, Ottavia and Ciutti, Arianna and Venditti, Vincenzo and Prischi, Filippo and Governatori, Mariangela and Bracci, Luisa and Lelli, Barbara and Pileri, Silvia and Botta, Mauro and Barge, Alessandro and Laschi, Franco and Niccolai, Neri (2006) NMR studies of BPTI aggregation by using paramagnetic relaxation reagents. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 1764 (5). pp. 856-862. DOI https://doi.org/10.1016/j.bbapap.2006.02.013
Bernini, Andrea and Spiga, Ottavia and Ciutti, Arianna and Venditti, Vincenzo and Prischi, Filippo and Governatori, Mariangela and Bracci, Luisa and Lelli, Barbara and Pileri, Silvia and Botta, Mauro and Barge, Alessandro and Laschi, Franco and Niccolai, Neri (2006) NMR studies of BPTI aggregation by using paramagnetic relaxation reagents. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 1764 (5). pp. 856-862. DOI https://doi.org/10.1016/j.bbapap.2006.02.013
Bernini, Andrea and Spiga, Ottavia and Ciutti, Arianna and Venditti, Vincenzo and Prischi, Filippo and Governatori, Mariangela and Bracci, Luisa and Lelli, Barbara and Pileri, Silvia and Botta, Mauro and Barge, Alessandro and Laschi, Franco and Niccolai, Neri (2006) NMR studies of BPTI aggregation by using paramagnetic relaxation reagents. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 1764 (5). pp. 856-862. DOI https://doi.org/10.1016/j.bbapap.2006.02.013
Abstract
Paramagnetic probes, whose approach to proteins can be monitored by nuclear magnetic resonance (NMR) studies, have been found of primary relevance for investigating protein surfaces accessibility. Here, paramagnetic probes are also suggested for a systematic investigation on protein aggregation. Bovin pancreatic trypsin inhibitor (BPTI) was used as a model system for aggregation by analyzing its interaction with TEMPOL and Gd(III)DTPA-BMA. Some of the measured paramagnetic relaxation rates of BPTI protons exhibited a reverse dependence on protein concentration, which can be attributed to the formation of transient BPTI aggregates. © 2006 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NMR; paramagnetic probe; TEMPOL; surface accessibility; Gd(III)DTPA-BMA; protein aggregation; BPTI |
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
| SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
| Depositing User: | Unnamed user with email elements@essex.ac.uk |
| Date Deposited: | 04 Feb 2016 12:57 |
| Last Modified: | 04 Dec 2024 06:26 |
| URI: | http://repository.essex.ac.uk/id/eprint/14842 |