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H4K16 acetylation marks active genes and enhancers of embryonic stem cells, but does not alter chromatin compaction

Taylor, Gillian CA and Eskeland, Ragnhild and Hekimoglu-Balkan, Betül and Pradeepa, Madapura M and Bickmore, Wendy A (2013) 'H4K16 acetylation marks active genes and enhancers of embryonic stem cells, but does not alter chromatin compaction.' Genome Research, 23 (12). pp. 2053-2065. ISSN 1088-9051

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<jats:p>Compared with histone H3, acetylation of H4 tails has not been well studied, especially in mammalian cells. Yet, H4K16 acetylation is of particular interest because of its ability to decompact nucleosomes in vitro and its involvement in dosage compensation in flies. Here we show that, surprisingly, loss of H4K16 acetylation does not alter higher-order chromatin compaction in vivo in mouse embryonic stem cells (ESCs). As well as peaks of acetylated H4K16 and KAT8 histone acetyltransferase at the transcription start sites of expressed genes, we report that acetylation of H4K16 is a new marker of active enhancers in ESCs and that some enhancers are marked by H3K4me1, KAT8, and H4K16ac, but not by acetylated H3K27 or EP300, suggesting that they are novel EP300 independent regulatory elements. Our data suggest a broad role for different histone acetylation marks and for different histone acetyltransferases in long-range gene regulation.</jats:p>

Item Type: Article
Uncontrolled Keywords: Cells, Cultured; Chromatin; Animals; Mice; Histones; Oligonucleotide Array Sequence Analysis; In Situ Hybridization, Fluorescence; Gene Expression Profiling; Epigenesis, Genetic; Gene Expression Regulation, Developmental; Acetylation; Transcription Initiation Site; Dosage Compensation, Genetic; E1A-Associated p300 Protein; Histone Acetyltransferases; Embryonic Stem Cells; Enhancer Elements, Genetic
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Science and Health
Faculty of Science and Health > Life Sciences, School of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 16 Oct 2015 11:13
Last Modified: 15 Jan 2022 00:49

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