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The Detection of Blur in Affymetrix GeneChips

Upton, Graham JG and Harrison, Andrew P (2010) 'The Detection of Blur in Affymetrix GeneChips.' Statistical Applications in Genetics and Molecular Biology, 9 (1). Article37-. ISSN 2194-6302

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High correlations were obtained between probes in seemingly unrelated probe sets, following an examination of the data from thousands of Affymetrix GeneChips. Investigation revealed that these unexpected correlations were between probes that were adjacent to high-valued probes. Using carefully selected probes, together with simple linear models, the extent of blur has been measured for each CEL file. The cause is shown to be attributable to poorly performing scanners. Blur can result in the doubling of the values of thousands of probes. This in turn can lead to the doubling of the expression level for hundreds of probe sets. Copyright © 2010 Berkeley Electronic Press.

Item Type: Article
Uncontrolled Keywords: Oligonucleotide Array Sequence Analysis; Data Interpretation, Statistical; Gene Expression Profiling
Subjects: H Social Sciences > HA Statistics
Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Science and Health
Faculty of Science and Health > Mathematical Sciences, Department of
SWORD Depositor: Elements
Depositing User: Elements
Date Deposited: 07 Sep 2011 13:40
Last Modified: 15 Jan 2022 00:45

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