Simkin, Andrew J and McAusland, Lorna and Headland, Lauren R and Lawson, Tracy and Raines, Christine A (2015) Multigene manipulation of photosynthetic carbon assimilation increases CO2 fixation and biomass yield in tobacco. Journal of Experimental Botany, 66 (13). pp. 4075-4090. DOI https://doi.org/10.1093/jxb/erv204
Simkin, Andrew J and McAusland, Lorna and Headland, Lauren R and Lawson, Tracy and Raines, Christine A (2015) Multigene manipulation of photosynthetic carbon assimilation increases CO2 fixation and biomass yield in tobacco. Journal of Experimental Botany, 66 (13). pp. 4075-4090. DOI https://doi.org/10.1093/jxb/erv204
Simkin, Andrew J and McAusland, Lorna and Headland, Lauren R and Lawson, Tracy and Raines, Christine A (2015) Multigene manipulation of photosynthetic carbon assimilation increases CO2 fixation and biomass yield in tobacco. Journal of Experimental Botany, 66 (13). pp. 4075-4090. DOI https://doi.org/10.1093/jxb/erv204
Abstract
Over the next 40 years it has been estimated that a 50% increase in the yield of grain crops such as wheat and rice will be required to meet the food and fuel demands of the increasing world population. Transgenic tobacco plants have been generated with altered combinations of sedoheptulose-1,7-bisphosphatase, fructose-1,6-bisphosphate aldolase, and the cyanobacterial putative-inorganic carbon transporter B, ictB, of which have all been identified as targets to improve photosynthesis based on empirical studies. It is shown here that increasing the levels of the three proteins individually significantly increases the rate of photosynthetic carbon assimilation, leaf area, and biomass yield. Furthermore, the daily integrated measurements of photosynthesis showed that mature plants fixed between 12-19% more CO2 than the equivalent wild-type plants. Further enhancement of photosynthesis and yield was observed when sedoheptulose-1,7-bisphosphatase, fructose-1,6-bisphosphate aldolase, and ictB were over-expressed together in the same plant. These results demonstrate the potential for the manipulation of photosynthesis, using multigene-stacking approaches, to increase crop yields.
Item Type: | Article |
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Uncontrolled Keywords: | Biomass; Calvin-Benson cycle; chlorophyll fluorescence imaging; gas exchange; gene stacking |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Arts and Humanities Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 18 May 2015 14:34 |
Last Modified: | 30 Oct 2024 19:33 |
URI: | http://repository.essex.ac.uk/id/eprint/13798 |
Available files
Filename: J. Exp. Bot.-2015-Simkin-jxb_erv204.pdf
Licence: Creative Commons: Attribution 3.0
Filename: Simkin et al JExBot Supp File.pdf