Steele, Deborah J and Franklin, Daniel J and Underwood, Graham JC (2014) Protection of cells from salinity stress by extracellular polymeric substances in diatom biofilms. Biofouling, 30 (8). pp. 987-998. DOI https://doi.org/10.1080/08927014.2014.960859
Steele, Deborah J and Franklin, Daniel J and Underwood, Graham JC (2014) Protection of cells from salinity stress by extracellular polymeric substances in diatom biofilms. Biofouling, 30 (8). pp. 987-998. DOI https://doi.org/10.1080/08927014.2014.960859
Steele, Deborah J and Franklin, Daniel J and Underwood, Graham JC (2014) Protection of cells from salinity stress by extracellular polymeric substances in diatom biofilms. Biofouling, 30 (8). pp. 987-998. DOI https://doi.org/10.1080/08927014.2014.960859
Abstract
Diatom biofilms are abundant in the marine environment. It is assumed (but untested) that extracellular polymeric substances (EPS), produced by diatoms, enable cells to cope with fluctuating salinity. To determine the protective role of EPS, Cylindrotheca closterium was grown in xanthan gum at salinities of 35, 50, 70 and 90 ppt. A xanthan matrix significantly increased cell viability (determined by SYTOX-Green), growth rate and population density by up to 300, 2,300 and 200%, respectively. Diatoms grown in 0.75% w/v xanthan, subjected to acute salinity shock treatments (at salinities 17.5, 50, 70 and 90 ppt) maintained photosynthetic capacity, Fq′/Fm′, within 4% of pre-shock values, whereas Fq′/Fm′ in cells grown without xanthan declined by up to 64% with hypersaline shock. Biofilms that developed in xanthan at standard salinity helped cells to maintain function during salinity shock. These results provide evidence of the benefits of living in an EPS matrix for biofilm diatoms.
Item Type: | Article |
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Uncontrolled Keywords: | biofilm; diatoms; Cylindrotheca; exopolymer; sea ice; salinity; xanthan gum |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 23 Jun 2015 13:37 |
Last Modified: | 30 Oct 2024 20:13 |
URI: | http://repository.essex.ac.uk/id/eprint/14007 |
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Filename: 08927014%2E2014%2E960859.pdf
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