Silaghi-Dumitrescu, Radu and Scurtu, Florina and Mason, Maria G and Svistunenko, Dimitri A and Wilson, Michael T and Cooper, Chris E (2015) The reaction of oxyhemoglobin with nitric oxide: EPR evidence for an iron(III)-nitrate intermediate. Inorganica Chimica Acta, 436. pp. 179-183. DOI https://doi.org/10.1016/j.ica.2015.07.037
Silaghi-Dumitrescu, Radu and Scurtu, Florina and Mason, Maria G and Svistunenko, Dimitri A and Wilson, Michael T and Cooper, Chris E (2015) The reaction of oxyhemoglobin with nitric oxide: EPR evidence for an iron(III)-nitrate intermediate. Inorganica Chimica Acta, 436. pp. 179-183. DOI https://doi.org/10.1016/j.ica.2015.07.037
Silaghi-Dumitrescu, Radu and Scurtu, Florina and Mason, Maria G and Svistunenko, Dimitri A and Wilson, Michael T and Cooper, Chris E (2015) The reaction of oxyhemoglobin with nitric oxide: EPR evidence for an iron(III)-nitrate intermediate. Inorganica Chimica Acta, 436. pp. 179-183. DOI https://doi.org/10.1016/j.ica.2015.07.037
Abstract
The reaction of oxyhemoglobin with nitric oxide is known to occur in vivo, with direct medical and pathophysiological relevance. The mechanism has been proposed to involve a transient iron(III)-peroxynitrite intermediate, the formation of which would be rate-limiting and the decay of which would yield iron(III)-aqua/iron(III)-hydroxo hemoglobin. Reported here are rapid-freeze-quench EPR (RFQ-EPR) spectroscopy data on the reaction of oxyhemoglobin with nitric oxide; no direct evidence is seen for an iron(III)-peroxynitrite intermediate. These findings are consistent with theoretical considerations according to which such an intermediate does form but is too short-lived to be detectable. Instead, iron(III) low-spin and high-spin transient species are detected in the RFQ-EPR experiments, which can be explained as arising from interactions of nitrate with methemoglobin.
Item Type: | Article |
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Uncontrolled Keywords: | Hemoglobin; Nitric oxide; Stopped-flow; Peroxynitrite; EPR |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 18 Aug 2015 12:49 |
Last Modified: | 30 Oct 2024 19:54 |
URI: | http://repository.essex.ac.uk/id/eprint/14560 |