Whitby, CB and Hall, G and Pickup, R and Saunders, JR and Ineson, P and Parekh, NR and McCarthy, A (2001) 13C incorporation into DNA as a means of identifying the active components of ammonia-oxidizer populations. Letters in Applied Microbiology, 32 (6). pp. 398-401. DOI https://doi.org/10.1046/j.1472-765x.2001.00930.x
Whitby, CB and Hall, G and Pickup, R and Saunders, JR and Ineson, P and Parekh, NR and McCarthy, A (2001) 13C incorporation into DNA as a means of identifying the active components of ammonia-oxidizer populations. Letters in Applied Microbiology, 32 (6). pp. 398-401. DOI https://doi.org/10.1046/j.1472-765x.2001.00930.x
Whitby, CB and Hall, G and Pickup, R and Saunders, JR and Ineson, P and Parekh, NR and McCarthy, A (2001) 13C incorporation into DNA as a means of identifying the active components of ammonia-oxidizer populations. Letters in Applied Microbiology, 32 (6). pp. 398-401. DOI https://doi.org/10.1046/j.1472-765x.2001.00930.x
Abstract
Aims: To identify active CO<inf>2</inf>-assimilating species of ammonia-oxidizing bacteria in fresh water sediment. Methods and Results: Enrichment cultures were incubated in the presence of <sup>13</sup>C labelled CO<inf>2</inf>, and <sup>13</sup>C-DNA successfully resolved from <sup>12</sup>C-DNA by caesium chloride density gradient ultracentrifugation of DNA extracts. Ammonia-oxidizer DNA recovered from these gradients was amplified and characterised by Temporal Temperature Gradient Gel Electrophoresis (TTGE), with confirmatory sequence analysis to identify the metabolically active components of the population. Conclusions: The <sup>12</sup>C-DNA fraction was dominated by nitrosospiras, in contrast to the <sup>13</sup>C-DNA fraction which was largely nitrosomonad DNA, in support of the hypothesis that nitrosomonads out-compete nitrosospiras in laboratory culture. Significance and Impact of the Study: The use of stable isotype incorporation into ammonia-oxidizer DNA could therefore circumvent the problems associated with RNA detection to identify metabolically active species in situ.
Item Type: | Article |
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Uncontrolled Keywords: | Nitrosomonas; Carbon Dioxide; Carbon Radioisotopes; DNA, Bacterial; Quaternary Ammonium Compounds |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 18 Jul 2017 08:56 |
Last Modified: | 11 Jun 2025 17:51 |
URI: | http://repository.essex.ac.uk/id/eprint/15842 |