Bradley, Justin M and Svistunenko, Dimitri A and Lawson, Tamara L and Hemmings, Andrew M and Moore, Geoffrey R and Le Brun, Nick E (2015) Three Aromatic Residues are Required for Electron Transfer during Iron Mineralization in Bacterioferritin. Angewandte Chemie International Edition, 54 (49). pp. 14763-14767. DOI https://doi.org/10.1002/anie.201507486
Bradley, Justin M and Svistunenko, Dimitri A and Lawson, Tamara L and Hemmings, Andrew M and Moore, Geoffrey R and Le Brun, Nick E (2015) Three Aromatic Residues are Required for Electron Transfer during Iron Mineralization in Bacterioferritin. Angewandte Chemie International Edition, 54 (49). pp. 14763-14767. DOI https://doi.org/10.1002/anie.201507486
Bradley, Justin M and Svistunenko, Dimitri A and Lawson, Tamara L and Hemmings, Andrew M and Moore, Geoffrey R and Le Brun, Nick E (2015) Three Aromatic Residues are Required for Electron Transfer during Iron Mineralization in Bacterioferritin. Angewandte Chemie International Edition, 54 (49). pp. 14763-14767. DOI https://doi.org/10.1002/anie.201507486
Abstract
<jats:title>Abstract</jats:title><jats:p>Ferritins are iron storage proteins that overcome the problems of toxicity and poor bioavailability of iron by catalyzing iron oxidation and mineralization through the activity of a diiron ferroxidase site. Unlike in other ferritins, the oxidized di‐Fe<jats:sup>3+</jats:sup> site of Escherichia coli bacterioferritin (EcBFR) is stable and therefore does not function as a conduit for the transfer of Fe<jats:sup>3+</jats:sup> into the storage cavity, but instead acts as a true catalytic cofactor that cycles its oxidation state while driving Fe<jats:sup>2+</jats:sup> oxidation in the cavity. Herein, we demonstrate that EcBFR mineralization depends on three aromatic residues near the diiron site, Tyr25, Tyr58, and Trp133, and that a transient radical is formed on Tyr25. The data indicate that the aromatic residues, together with a previously identified inner surface iron site, promote mineralization by ensuring the simultaneous delivery of two electrons, derived from Fe<jats:sup>2+</jats:sup> oxidation in the BFR cavity, to the di‐ferric catalytic site for safe reduction of O<jats:sub>2</jats:sub>.</jats:p>
Item Type: | Article |
---|---|
Uncontrolled Keywords: | bioinorganic chemistry; ferritin; iron; mineralization; tyrosyl radicals |
Subjects: | Q Science > QD Chemistry |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 28 Jan 2016 16:49 |
Last Modified: | 30 Oct 2024 20:21 |
URI: | http://repository.essex.ac.uk/id/eprint/15992 |
Available files
Filename: Bradley_et_al-2015-Angewandte_Chemie_International_Edition.pdf
Licence: Creative Commons: Attribution 3.0