Coulon, Frédéric and McKew, Boyd A and Osborn, A Mark and McGenity, Terry J and Timmis, Kenneth N (2007) Effects of temperature and biostimulation on oil‐degrading microbial communities in temperate estuarine waters. Environmental Microbiology, 9 (1). pp. 177-186. DOI https://doi.org/10.1111/j.1462-2920.2006.01126.x
Coulon, Frédéric and McKew, Boyd A and Osborn, A Mark and McGenity, Terry J and Timmis, Kenneth N (2007) Effects of temperature and biostimulation on oil‐degrading microbial communities in temperate estuarine waters. Environmental Microbiology, 9 (1). pp. 177-186. DOI https://doi.org/10.1111/j.1462-2920.2006.01126.x
Coulon, Frédéric and McKew, Boyd A and Osborn, A Mark and McGenity, Terry J and Timmis, Kenneth N (2007) Effects of temperature and biostimulation on oil‐degrading microbial communities in temperate estuarine waters. Environmental Microbiology, 9 (1). pp. 177-186. DOI https://doi.org/10.1111/j.1462-2920.2006.01126.x
Abstract
<jats:title>Summary</jats:title><jats:p>Improved strategies for oil‐spill remediation will follow a better understanding of the nature, activities and regulating parameters of petroleum hydrocarbon‐degrading microbial communities in temperate marine environments. The addition of crude oil to estuarine water resulted in an immediate change in bacterial community structure, increased abundance of hydrocarbon‐degrading microorganisms and a rapid rate of oil degradation, suggesting the presence of a pre‐adapted oil‐degrading microbial community and sufficient supply of nutrients. Relatively rapid degradation was found at 4°C, the lowest temperature tested; and it was temperature rather than nutrient addition that most influenced the community structure. A detailed phylogenetic analysis of oil‐degrading microcosms showed that known hydrocarbonoclastic organisms like <jats:italic>Thalassolituus</jats:italic> and <jats:italic>Cycloclasticus</jats:italic>, as well as proposed oil degraders like <jats:italic>Roseobacter</jats:italic>, were present at both 4°C and 20°C, demonstrating the thermo‐versatility of such organisms. Clones related to <jats:italic>Oleispira antarctica</jats:italic> (98% 16S rRNA similarity), a psychrophilic alkane degrader, were dominant in the 4°C oil‐degrading community, whereas other clones constituting a different clade and showing 94% similarity 16S rRNA with <jats:italic>O. antarctica</jats:italic> were found <jats:italic>in situ.</jats:italic> These findings demonstrate the potential for intrinsic bioremediation throughout the course of the year in temperate estuarine waters, and highlight the importance of both versatile psychrotolerant and specialized psychrophilic hydrocarbon‐degrading microbes in effecting this process at low temperatures.</jats:p>
Item Type: | Article |
---|---|
Uncontrolled Keywords: | Bacteria; DNA, Ribosomal; RNA, Ribosomal, 16S; Water Pollutants, Chemical; Colony Count, Microbial; Ecosystem; Petroleum; Temperature; Seawater; Phylogeny; Geologic Sediments; Biodegradation, Environmental |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 01 Jul 2016 14:49 |
Last Modified: | 30 Oct 2024 19:48 |
URI: | http://repository.essex.ac.uk/id/eprint/17153 |