Nikolaeva, Marina A and Babayan, Alina A and Stepanova, Elena O and Smolnikova, Veronika Y and Kalinina, Elena A and Fernández, Nelson and Krechetova, Lubov V and Vanko, Ludmila V and Sukhikh, Gennady T (2016) The relationship of seminal transforming growth factor-β1 and interleukin-18 with reproductive success in women exposed to seminal plasma during IVF/ICSI treatment. Journal of Reproductive Immunology, 117. pp. 45-51. DOI https://doi.org/10.1016/j.jri.2016.03.006
Nikolaeva, Marina A and Babayan, Alina A and Stepanova, Elena O and Smolnikova, Veronika Y and Kalinina, Elena A and Fernández, Nelson and Krechetova, Lubov V and Vanko, Ludmila V and Sukhikh, Gennady T (2016) The relationship of seminal transforming growth factor-β1 and interleukin-18 with reproductive success in women exposed to seminal plasma during IVF/ICSI treatment. Journal of Reproductive Immunology, 117. pp. 45-51. DOI https://doi.org/10.1016/j.jri.2016.03.006
Nikolaeva, Marina A and Babayan, Alina A and Stepanova, Elena O and Smolnikova, Veronika Y and Kalinina, Elena A and Fernández, Nelson and Krechetova, Lubov V and Vanko, Ludmila V and Sukhikh, Gennady T (2016) The relationship of seminal transforming growth factor-β1 and interleukin-18 with reproductive success in women exposed to seminal plasma during IVF/ICSI treatment. Journal of Reproductive Immunology, 117. pp. 45-51. DOI https://doi.org/10.1016/j.jri.2016.03.006
Abstract
It has been proposed that the transforming growth factor (TGF)-β1 present in seminal plasma (SP) triggers a female immune response favorable for implantation. We hypothesize that seminal interleukin (IL)-18, a cytokine that can potentially cause implantation failure, interferes with the beneficial effect of TGF-β1. This study aims to determine whether the levels of seminal TGF-β1 and IL-18 are associated with reproductive outcomes in patients exposed to SP during in vitro fertilization (IVF) or IVF with intracytoplasmic sperm injection (ICSI). A prospective study, which included 71 couples undergoing IVF/ICSI was carried out. Female patients were exposed to their partners’ SP via timed intercourse before the day of ovum pick-up (OPU) and also subjected to intravaginal SP application just after OPU. Quantitative measurements of total TGF-β1 (active plus latent) as well as IL-18 were determined by FlowCytomix™ technology in the SP to be used for intravaginal applications. Comparison of SP cytokine profiles between pregnant and non-pregnant groups revealed that pregnancy was correlated with a lower concentration of IL-18 (P = 0.018) and lower content per ejaculate for both of IL-18 (P = 0.0003) and TGF-β1 (P = 0.047). The ratio of TGF-β1-to-IL-18 concentration was significantly higher in the pregnant than in the non-pregnant group (P = 0.026). This study supports the notion that two key cytokines TGF-β1 and IL-18, both present in SP are associated with reproductive outcomes in female patients exposed to SP during IVF/ICSI treatment.
Item Type: | Article |
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Uncontrolled Keywords: | IVF/ICSI outcome; Interleukin-18; Seminal plasma; Transforming growth factor-beta 1 |
Subjects: | Q Science > QH Natural history > QH301 Biology R Medicine > RG Gynecology and obstetrics |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 12 Jul 2016 08:25 |
Last Modified: | 07 Aug 2024 19:48 |
URI: | http://repository.essex.ac.uk/id/eprint/17238 |