Howard, Thomas P and Upton, Graham JG and Lloyd, Julie C and Raines, Christine A (2011) Antisense suppression of the small chloroplast protein CP12 in tobacco: A transcriptional viewpoint. Plant Signaling & Behavior, 6 (12). pp. 2026-2030. DOI https://doi.org/10.4161/psb.6.12.18055
Howard, Thomas P and Upton, Graham JG and Lloyd, Julie C and Raines, Christine A (2011) Antisense suppression of the small chloroplast protein CP12 in tobacco: A transcriptional viewpoint. Plant Signaling & Behavior, 6 (12). pp. 2026-2030. DOI https://doi.org/10.4161/psb.6.12.18055
Howard, Thomas P and Upton, Graham JG and Lloyd, Julie C and Raines, Christine A (2011) Antisense suppression of the small chloroplast protein CP12 in tobacco: A transcriptional viewpoint. Plant Signaling & Behavior, 6 (12). pp. 2026-2030. DOI https://doi.org/10.4161/psb.6.12.18055
Abstract
The chloroplast protein CP12 forms a multi-enzyme complex with the Calvin-Benson cycle enzymes phosphoribulokinase (PRK) and NADP-glyceraldehyde-3-phosphate dehydrogenase (GAPDH). PRK and GAPDH are inactivated when present in this complex, a process shown in vitro to be dependent upon oxidized CP12. Recently we reported on the importance of CP12 in vivo to higher plant metabolism using antisense suppression of CP12 in tobacco (Nicotiana tabacum). Our results indicated that while only minor changes in photosynthetic carbon fixation and in PRK and GAPDH activities were observed, striking changes in growth rates and morphology were seen. In this article we present data on the transcriptional changes observed in one of the antisense lines and we discuss the major findings in light of the metabolic phenotype described. © 2011 Landes Bioscience.
Item Type: | Article |
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Uncontrolled Keywords: | Phosphotransferases (Alcohol Group Acceptor); Plant Proteins; Antisense Elements (Genetics); Gene Expression Profiling; Photosynthesis; Transcription, Genetic; Gene Expression Regulation, Plant; Gene Silencing; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); Transcriptome; Chloroplast Proteins; Nicotiana |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 10 Jan 2017 10:29 |
Last Modified: | 23 Oct 2024 05:08 |
URI: | http://repository.essex.ac.uk/id/eprint/18781 |