Zaichick, Sofia V and Metodiev, Metodi V and Nelson, Scott A and Durbrovskyi, Oleksii and Draper, Edward and Cooper, John A and Stone, David E (2009) The Mating-specific Gα Interacts with a Kinesin-14 and Regulates Pheromone-induced Nuclear Migration in Budding Yeast. Molecular Biology of the Cell, 20 (12). pp. 2820-2830. DOI https://doi.org/10.1091/mbc.e09-01-0069
Zaichick, Sofia V and Metodiev, Metodi V and Nelson, Scott A and Durbrovskyi, Oleksii and Draper, Edward and Cooper, John A and Stone, David E (2009) The Mating-specific Gα Interacts with a Kinesin-14 and Regulates Pheromone-induced Nuclear Migration in Budding Yeast. Molecular Biology of the Cell, 20 (12). pp. 2820-2830. DOI https://doi.org/10.1091/mbc.e09-01-0069
Zaichick, Sofia V and Metodiev, Metodi V and Nelson, Scott A and Durbrovskyi, Oleksii and Draper, Edward and Cooper, John A and Stone, David E (2009) The Mating-specific Gα Interacts with a Kinesin-14 and Regulates Pheromone-induced Nuclear Migration in Budding Yeast. Molecular Biology of the Cell, 20 (12). pp. 2820-2830. DOI https://doi.org/10.1091/mbc.e09-01-0069
Abstract
<jats:p>As a budding yeast cell elongates toward its mating partner, cytoplasmic microtubules connect the nucleus to the cell cortex at the growth tip. The Kar3 kinesin-like motor protein is then thought to stimulate plus-end depolymerization of these microtubules, thus drawing the nucleus closer to the site where cell fusion and karyogamy will occur. Here, we show that pheromone stimulates a microtubule-independent interaction between Kar3 and the mating-specific Gα protein Gpa1 and that Gpa1 affects both microtubule orientation and cortical contact. The membrane localization of Gpa1 was found to polarize early in the mating response, at about the same time that the microtubules begin to attach to the incipient growth site. In the absence of Gpa1, microtubules lose contact with the cortex upon shrinking and Kar3 is improperly localized, suggesting that Gpa1 is a cortical anchor for Kar3. We infer that Gpa1 serves as a positional determinant for Kar3-bound microtubule plus ends during mating.</jats:p>
Item Type: | Article |
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Uncontrolled Keywords: | Cell Nucleus; Microtubules; Saccharomyces cerevisiae; Actins; Microtubule Proteins; GTP-Binding Protein alpha Subunits, Gq-G11; Microtubule-Associated Proteins; Saccharomyces cerevisiae Proteins; Green Fluorescent Proteins; Recombinant Fusion Proteins; Pheromones; Fluorescence Recovery After Photobleaching; Cell Polarity; Protein Binding; Biological Transport; Genes, Mating Type, Fungal |
Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 14 Jun 2012 09:13 |
Last Modified: | 30 Oct 2024 16:43 |
URI: | http://repository.essex.ac.uk/id/eprint/2477 |
Available files
Filename: kar3_paper.pdf