Ermakova, Maria and Lopez-Calcagno, Patricia E and Raines, Christine A and Furbank, Robert T and von Caemmerer, Susanne (2019) Overexpression of the Rieske FeS protein of the Cytochrome b 6 f complex increases C4 photosynthesis in Setaria viridis. Communications Biology, 2 (1). 314-. DOI https://doi.org/10.1038/s42003-019-0561-9
Ermakova, Maria and Lopez-Calcagno, Patricia E and Raines, Christine A and Furbank, Robert T and von Caemmerer, Susanne (2019) Overexpression of the Rieske FeS protein of the Cytochrome b 6 f complex increases C4 photosynthesis in Setaria viridis. Communications Biology, 2 (1). 314-. DOI https://doi.org/10.1038/s42003-019-0561-9
Ermakova, Maria and Lopez-Calcagno, Patricia E and Raines, Christine A and Furbank, Robert T and von Caemmerer, Susanne (2019) Overexpression of the Rieske FeS protein of the Cytochrome b 6 f complex increases C4 photosynthesis in Setaria viridis. Communications Biology, 2 (1). 314-. DOI https://doi.org/10.1038/s42003-019-0561-9
Abstract
C4 photosynthesis is characterised by a CO2 concentrating mechanism that operates between mesophyll and bundle sheath cells increasing CO2 partial pressure at the site of Rubisco and photosynthetic efficiency. Electron transport chains in both cell types supply ATP and NADPH for C4 photosynthesis. Cytochrome b 6 f is a key control point of electron transport in C3 plants. To study whether C4 photosynthesis is limited by electron transport we constitutively overexpressed the Rieske FeS subunit in Setaria viridis. This resulted in a higher Cytochrome b 6 f content in mesophyll and bundle sheath cells without marked changes in the abundances of other photosynthetic proteins. Rieske overexpression plants showed better light conversion efficiency in both Photosystems and could generate higher proton-motive force across the thylakoid membrane underpinning an increase in CO2 assimilation rate at ambient and saturating CO2 and high light. Our results demonstrate that removing electron transport limitations can increase C4 photosynthesis.
Item Type: | Article |
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Uncontrolled Keywords: | Plants, Genetically Modified; Setaria Plant; Carbon Dioxide; Cytochrome b6f Complex; Electron Transport Complex III; Plant Proteins; Photosynthesis; Gene Expression Regulation, Plant; Proton-Motive Force; Fluorescence |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 05 May 2020 17:26 |
Last Modified: | 30 Oct 2024 16:19 |
URI: | http://repository.essex.ac.uk/id/eprint/27448 |
Available files
Filename: Overexpression of the Rieske FeS protein of the Cytochrome b 6 f complex increases C4 photosynthesis in Setaria viridis.pdf
Licence: Creative Commons: Attribution 3.0