McLachlan, Deirdre H and Underwood, Graham JC and Taylor, Alison R and Brownlee, Colin (2012) CALCIUM RELEASE FROM INTRACELLULAR STORES IS NECESSARY FOR THE PHOTOPHOBIC RESPONSE IN THE BENTHIC DIATOM <i>NAVICULA PERMINUTA</i> (BACILLARIOPHYCEAE)<sup>1</sup>. Journal of Phycology, 48 (3). pp. 675-681. DOI https://doi.org/10.1111/j.1529-8817.2012.01158.x
McLachlan, Deirdre H and Underwood, Graham JC and Taylor, Alison R and Brownlee, Colin (2012) CALCIUM RELEASE FROM INTRACELLULAR STORES IS NECESSARY FOR THE PHOTOPHOBIC RESPONSE IN THE BENTHIC DIATOM <i>NAVICULA PERMINUTA</i> (BACILLARIOPHYCEAE)<sup>1</sup>. Journal of Phycology, 48 (3). pp. 675-681. DOI https://doi.org/10.1111/j.1529-8817.2012.01158.x
McLachlan, Deirdre H and Underwood, Graham JC and Taylor, Alison R and Brownlee, Colin (2012) CALCIUM RELEASE FROM INTRACELLULAR STORES IS NECESSARY FOR THE PHOTOPHOBIC RESPONSE IN THE BENTHIC DIATOM <i>NAVICULA PERMINUTA</i> (BACILLARIOPHYCEAE)<sup>1</sup>. Journal of Phycology, 48 (3). pp. 675-681. DOI https://doi.org/10.1111/j.1529-8817.2012.01158.x
Abstract
<jats:p>Complex photoreceptor pathways exist in algae to exploit light as a sensory stimulus. Previous studies have implicated calcium in blue‐light signaling in plants and algae. A photophobic response to high‐intensity blue light was characterized in the marine benthic diatom <jats:italic>Navicula perminuta</jats:italic> (Grunow) in van Heurck. Calcium modulators were used to determine the involvement of calcium in the signaling of this response, and the fluorescent calcium indicator Calcium Crimson was used to image changes in intracellular [Ca<jats:sup>2+</jats:sup>] during a response. A localized, transient elevation of Calcium Crimson fluorescence was seen at the cell tip at the time of cell reversal. Intracellular calcium release inhibitors produced a significant decrease in the population photophobic response. Treatments known to decrease influx of extracellular calcium had no effect on the population photophobic response but did cause a significant decrease in average cell speed. As the increase in intracellular [Ca<jats:sup>2+</jats:sup>] at the cell tip corresponded to the time of direction change rather than the onset of the light stimulus, it would appear that Ca<jats:sup>2+</jats:sup> constitutes a component of the switching mechanism that leads to reversal of the locomotion machinery. Our current evidence suggests that the source of this Ca<jats:sup>2+</jats:sup> is intracellular.</jats:p>
Item Type: | Article |
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Uncontrolled Keywords: | blue light; calcium; diatom; motility; signaling |
Subjects: | Q Science > QH Natural history Q Science > QH Natural history > QH301 Biology Q Science > QK Botany Q Science > QR Microbiology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 24 Apr 2013 13:59 |
Last Modified: | 30 Oct 2024 20:11 |
URI: | http://repository.essex.ac.uk/id/eprint/5929 |