Bechtold, Ulrike and Rabbani, Naila and Mullineaux, Philip M and Thornalley, Paul J (2009) Quantitative measurement of specific biomarkers for protein oxidation, nitration and glycation in Arabidopsis leaves. The Plant Journal, 59 (4). pp. 661-671. DOI https://doi.org/10.1111/j.1365-313x.2009.03898.x
Bechtold, Ulrike and Rabbani, Naila and Mullineaux, Philip M and Thornalley, Paul J (2009) Quantitative measurement of specific biomarkers for protein oxidation, nitration and glycation in Arabidopsis leaves. The Plant Journal, 59 (4). pp. 661-671. DOI https://doi.org/10.1111/j.1365-313x.2009.03898.x
Bechtold, Ulrike and Rabbani, Naila and Mullineaux, Philip M and Thornalley, Paul J (2009) Quantitative measurement of specific biomarkers for protein oxidation, nitration and glycation in Arabidopsis leaves. The Plant Journal, 59 (4). pp. 661-671. DOI https://doi.org/10.1111/j.1365-313x.2009.03898.x
Abstract
<jats:title>Summary</jats:title><jats:p>Higher plants are continually exposed to reactive oxygen and nitrogen species during their lives. Together with glucose and reactive dicarbonyls, these can modify proteins spontaneously, leading to protein oxidation, nitration and glycation. These reactions have the potential to damage proteins and have an impact on physiological processes. The levels of protein oxidation, nitration and glycation adducts were assayed, using liquid chromatography coupled with tandem mass spectrometry, in total leaf extracts over a diurnal cycle and when exposed to conditions that promote oxidative stress. Changes in the levels of oxidation, glycation and nitration adducts were found between the light and dark phases under non‐stress conditions. A comparison between wild‐type plants and a mutant lacking peptide methionine sulfoxide reductase (<jats:italic>pmsr2‐1</jats:italic>) showed increased protein oxidation, nitration and glycation of specific amino acid residues during darkness in <jats:italic>pmsr2‐1</jats:italic>. Short‐term excess light exposure, which promoted oxidative stress, led to increased protein glycation, specifically by glyoxal. This suggested that any increased oxidative damage to proteins was within the repair capacity of the plant. The methods developed here provide the means to simultaneously detect a range of protein oxidation, nitration and glycation adducts within a single sample. Thus, these methods identify a range of biomarkers to monitor a number of distinct biochemical processes that have an impact on the proteome and therefore the physiological state of the plant.</jats:p>
Item Type: | Article |
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Uncontrolled Keywords: | Arabidopsis; Plant Leaves; Arabidopsis Proteins; Oxidation-Reduction; Glycosylation; Oxidative Stress; Light; Photoperiod; Tandem Mass Spectrometry; Biomarkers |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of Faculty of Science and Health > Computer Science and Electronic Engineering, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 16 Sep 2011 09:00 |
Last Modified: | 30 Oct 2024 19:38 |
URI: | http://repository.essex.ac.uk/id/eprint/686 |