Shaikly, Valerie R and Morrison, Ian EG and Taranissi, Mohamed and Noble, Clare V and Withey, Anna D and Cherry, Richard J and Blois, Sandra M and Fernández, Nelson (2008) Analysis of HLA-G in Maternal Plasma, Follicular Fluid, and Preimplantation Embryos Reveal an Asymmetric Pattern of Expression. The Journal of Immunology, 180 (6). pp. 4330-4337. DOI https://doi.org/10.4049/jimmunol.180.6.4330
Shaikly, Valerie R and Morrison, Ian EG and Taranissi, Mohamed and Noble, Clare V and Withey, Anna D and Cherry, Richard J and Blois, Sandra M and Fernández, Nelson (2008) Analysis of HLA-G in Maternal Plasma, Follicular Fluid, and Preimplantation Embryos Reveal an Asymmetric Pattern of Expression. The Journal of Immunology, 180 (6). pp. 4330-4337. DOI https://doi.org/10.4049/jimmunol.180.6.4330
Shaikly, Valerie R and Morrison, Ian EG and Taranissi, Mohamed and Noble, Clare V and Withey, Anna D and Cherry, Richard J and Blois, Sandra M and Fernández, Nelson (2008) Analysis of HLA-G in Maternal Plasma, Follicular Fluid, and Preimplantation Embryos Reveal an Asymmetric Pattern of Expression. The Journal of Immunology, 180 (6). pp. 4330-4337. DOI https://doi.org/10.4049/jimmunol.180.6.4330
Abstract
<jats:title>Abstract</jats:title> <jats:p>Soluble HLA-G (sHLA-G) secretion by human preimplantation embryos in culture has been associated with successful embryo development, and therefore has potential to serve as a noninvasive marker of embryo viability. We have examined the spatial and temporal expression of HLA-G in embryos of varying developmental competence and the role of maternal factors in human embryonic HLA-G expression. Embryos that reached blastocyst stage on day 5 showed a higher frequency of sHLA-G secretion than those at morula or arrested stages (p &lt; 0.05). There was no significant difference in sHLA-G secretion between normal embryos and those diagnosed as chromosomally abnormal by preimplantation genetic diagnosis. HLA-G detected in maternal plasma and follicular fluid did not appear to correlate with HLA-G expressed in the embryo or embryo supernatants. Confocal microscopy analysis indicated that HLA-G protein expression in embryos was not homogeneous; mostly, it was confined to blastocysts localized on trophectoderm and trophectoderm projections. Single-particle fluorescent imaging analysis of HLA-G on the cell surface of JEG-3 cells showed that HLA-G particles were mostly monomeric, but dimeric and higher order oligomers were also observed. These results suggest that HLA-G play an important role in preimplantation embryo development. However, the observed expression of HLA-G in arrested and chromosomally abnormal embryos indicates that HLA-G testing should be used with caution and in conjunction with conventional methods of embryo screening and selection.</jats:p>
Item Type: | Article |
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Uncontrolled Keywords: | Follicular Fluid; Ovum; Cell Line, Tumor; Cell-Free System; Blastocyst; Humans; Histocompatibility Antigens Class I; HLA Antigens; Microscopy, Confocal; Microscopy, Fluorescence; Preimplantation Diagnosis; Embryo Culture Techniques; Pregnancy; Maternal-Fetal Exchange; Female; HLA-G Antigens |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 16 Sep 2011 09:03 |
Last Modified: | 02 Nov 2024 02:50 |
URI: | http://repository.essex.ac.uk/id/eprint/714 |