Abdel-Majid, Raja M and Leong, Wey L and Schalkwyk, Leonard C and Smallman, Donald S and Wong, Scott T and Storm, Daniel R and Fine, Alan and Dobson, Melanie J and Guernsey, Duane L and Neumann, Paul E (1998) Loss of adenylyl cyclase I activity disrupts patterning of mouse somatosensory cortex. Nature Genetics, 19 (3). pp. 289-291. DOI https://doi.org/10.1038/980
Abdel-Majid, Raja M and Leong, Wey L and Schalkwyk, Leonard C and Smallman, Donald S and Wong, Scott T and Storm, Daniel R and Fine, Alan and Dobson, Melanie J and Guernsey, Duane L and Neumann, Paul E (1998) Loss of adenylyl cyclase I activity disrupts patterning of mouse somatosensory cortex. Nature Genetics, 19 (3). pp. 289-291. DOI https://doi.org/10.1038/980
Abdel-Majid, Raja M and Leong, Wey L and Schalkwyk, Leonard C and Smallman, Donald S and Wong, Scott T and Storm, Daniel R and Fine, Alan and Dobson, Melanie J and Guernsey, Duane L and Neumann, Paul E (1998) Loss of adenylyl cyclase I activity disrupts patterning of mouse somatosensory cortex. Nature Genetics, 19 (3). pp. 289-291. DOI https://doi.org/10.1038/980
Abstract
The somatosensory (SI) cortex of mice displays a patterned, nonuniform distribution of neurons in layer IV called the 'barrelfield' (ref. 1). Thalamocortical afferents (TCAs) that terminate in layer IV are segregated such that each barrel, a readily visible cylindrical array of neurons surrounding a cell-sparse center, represents a distinct receptive field. TCA arbors are confined to the barrel hollow and synapse on barrel-wall neurons whose dendrites are oriented toward the center of the barrel. Mice homozygous for the barrelless (brl) mutation, which occurred spontaneously in ICR stock at Universite de Lausanne (Switzerland), fail to develop this patterned distribution of neurons, but still display normal topological organization of the SI cortex. Despite the absence of barrels and the overlapping zones of TCA arborization, the size of individual whisker representations, as judged by 2-deoxyglucose uptake, is similar to that of wild-type mice. We identified adenylyl cyclase type I (Adcy1) as the gene disrupted in brl mutant mice by fine mapping of proximal chromosome 11, enzyme assay, mutation analysis and examination of mice homozygous for a targeted disruption of Adcy1. These results provide the first evidence for involvement of cAMP signalling pathways in pattern formation of the brain.
Item Type: | Article |
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Uncontrolled Keywords: | Brain; Somatosensory Cortex; Neurons; Animals; Mice, Inbred C57BL; Mice, Knockout; Mice; Membrane Proteins; DNA, Complementary; Base Sequence; Body Patterning; Molecular Sequence Data; Female; Male; Adenylyl Cyclases |
Subjects: | Q Science > QH Natural history > QH426 Genetics |
Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
Depositing User: | Unnamed user with email elements@essex.ac.uk |
Date Deposited: | 17 Jul 2017 12:37 |
Last Modified: | 07 Aug 2024 20:26 |
URI: | http://repository.essex.ac.uk/id/eprint/10979 |