Gösele, Claudia and Hong, Liu and Kreitler, Thomas and Rossmann, Marlies and Hieke, Brigitte and Groß, Uwe and Kramer, Markus and Himmelbauer, Heinz and Bihoreau, Marie-Thérese and Kwitek-Black, Anne E and Twigger, Simon and Tonellato, Peter J and Jacob, Howard J and Schalkwyk, Leonard C and Lindpaintner, Klaus and Ganten, Detlev and Lehrach, Hans and Knoblauch, Margit (2000) High-Throughput Scanning of the Rat Genome Using Interspersed Repetitive Sequence-PCR Markers. Genomics, 69 (3). pp. 287-294. DOI https://doi.org/10.1006/geno.2000.6352
Gösele, Claudia and Hong, Liu and Kreitler, Thomas and Rossmann, Marlies and Hieke, Brigitte and Groß, Uwe and Kramer, Markus and Himmelbauer, Heinz and Bihoreau, Marie-Thérese and Kwitek-Black, Anne E and Twigger, Simon and Tonellato, Peter J and Jacob, Howard J and Schalkwyk, Leonard C and Lindpaintner, Klaus and Ganten, Detlev and Lehrach, Hans and Knoblauch, Margit (2000) High-Throughput Scanning of the Rat Genome Using Interspersed Repetitive Sequence-PCR Markers. Genomics, 69 (3). pp. 287-294. DOI https://doi.org/10.1006/geno.2000.6352
Gösele, Claudia and Hong, Liu and Kreitler, Thomas and Rossmann, Marlies and Hieke, Brigitte and Groß, Uwe and Kramer, Markus and Himmelbauer, Heinz and Bihoreau, Marie-Thérese and Kwitek-Black, Anne E and Twigger, Simon and Tonellato, Peter J and Jacob, Howard J and Schalkwyk, Leonard C and Lindpaintner, Klaus and Ganten, Detlev and Lehrach, Hans and Knoblauch, Margit (2000) High-Throughput Scanning of the Rat Genome Using Interspersed Repetitive Sequence-PCR Markers. Genomics, 69 (3). pp. 287-294. DOI https://doi.org/10.1006/geno.2000.6352
Abstract
We report the establishment of a hybridization-based marker system for the rat genome based on the PCR amplification of interspersed repetitive sequences (IRS). Overall, 351 IRS markers were mapped within the rat genome. The IRS marker panel consists of 210 nonpolymorphic and 141 polymorphic markers that were screened for presence/absence polymorphism patterns in 38 different rat strains and substrains that are commonly used in biomedical research. The IRS marker panel was demonstrated to be useful for rapid genome screening in experimental rat crosses and high-throughput characterization of large-insert genomic library clones. Information on corresponding YAC clones is made available for this IRS marker set distributed over the whole rat genome. The two existing rat radiation hybrid maps were integrated by placing the IRS markers in both maps. The genetic and physical mapping data presented provide substantial information for ongoing positional cloning projects in the rat. (C) 2000 Academic Press.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Cell Line; Chromosomes, Artificial, Yeast; Animals; Rats, Inbred Strains; Rats, Inbred F344; Rats; Genetic Markers; Chromosome Mapping; Polymerase Chain Reaction; Interspersed Repetitive Sequences; Genome; Cricetinae |
| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
| SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
| Depositing User: | Unnamed user with email elements@essex.ac.uk |
| Date Deposited: | 02 Aug 2017 12:10 |
| Last Modified: | 18 Aug 2022 11:41 |
| URI: | http://repository.essex.ac.uk/id/eprint/11030 |