Scotti, John S and Leung, Ivanhoe KH and Ge, Wei and Bentley, Michael A and Paps, Jordi and Kramer, Holger B and Lee, Joongoo and Aik, WeiShen and Choi, Hwanho and Paulsen, Steinar M and Bowman, Lesley AH and Loik, Nikita D and Horita, Shoichiro and Ho, Chia-hua and Kershaw, Nadia J and Tang, Christoph M and Claridge, Timothy DW and Preston, Gail M and McDonough, Michael A and Schofield, Christopher J (2014) Human oxygen sensing may have origins in prokaryotic elongation factor Tu prolyl-hydroxylation. Proceedings of the National Academy of Sciences, 111 (37). pp. 13331-13336. DOI https://doi.org/10.1073/pnas.1409916111
Scotti, John S and Leung, Ivanhoe KH and Ge, Wei and Bentley, Michael A and Paps, Jordi and Kramer, Holger B and Lee, Joongoo and Aik, WeiShen and Choi, Hwanho and Paulsen, Steinar M and Bowman, Lesley AH and Loik, Nikita D and Horita, Shoichiro and Ho, Chia-hua and Kershaw, Nadia J and Tang, Christoph M and Claridge, Timothy DW and Preston, Gail M and McDonough, Michael A and Schofield, Christopher J (2014) Human oxygen sensing may have origins in prokaryotic elongation factor Tu prolyl-hydroxylation. Proceedings of the National Academy of Sciences, 111 (37). pp. 13331-13336. DOI https://doi.org/10.1073/pnas.1409916111
Scotti, John S and Leung, Ivanhoe KH and Ge, Wei and Bentley, Michael A and Paps, Jordi and Kramer, Holger B and Lee, Joongoo and Aik, WeiShen and Choi, Hwanho and Paulsen, Steinar M and Bowman, Lesley AH and Loik, Nikita D and Horita, Shoichiro and Ho, Chia-hua and Kershaw, Nadia J and Tang, Christoph M and Claridge, Timothy DW and Preston, Gail M and McDonough, Michael A and Schofield, Christopher J (2014) Human oxygen sensing may have origins in prokaryotic elongation factor Tu prolyl-hydroxylation. Proceedings of the National Academy of Sciences, 111 (37). pp. 13331-13336. DOI https://doi.org/10.1073/pnas.1409916111
Abstract
<jats:title>Significance</jats:title><jats:p>The Fe(II)- and 2-oxoglutarate (2OG)-dependent hypoxia-inducible transcription factor prolyl-hydroxylases play a central role in human oxygen sensing and are related to other prolyl-hydroxylases involved in eukaryotic collagen biosynthesis and ribosomal modification. The finding that a PHD-related prolyl-hydroxylase in<jats:italic>Pseudomonas spp.</jats:italic>regulates pyocyanin biosynthesis supports prokaryotic origins for the eukaryotic prolyl-hydroxylases. The identification of the switch I loop of elongation factor Tu (EF-Tu) as a<jats:italic>Pseudomonas</jats:italic>prolyl-hydroxylase domain containing protein (PPHD) substrate provides evidence of roles for 2OG oxygenases in both translational and transcriptional regulation. A structure of the PPHD:EF-Tu complex, the first to the authors' knowledge of a 2OG oxygenase with its intact protein substrate, reveals that major conformational changes occur in both PPHD and EF-Tu and will be useful in the design of new prolyl-hydroxylase inhibitors.</jats:p>
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Humans; Chlamydomonas reinhardtii; Pseudomonas putida; Oxygen; Peptide Elongation Factor Tu; Proline; Protein Structure, Secondary; Protein Structure, Tertiary; Substrate Specificity; Hydroxylation; Models, Molecular; Molecular Sequence Data; Hypoxia-Inducible Factor 1, alpha Subunit; Hypoxia-Inducible Factor-Proline Dioxygenases |
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics |
| Divisions: | Faculty of Science and Health Faculty of Science and Health > Life Sciences, School of |
| SWORD Depositor: | Unnamed user with email elements@essex.ac.uk |
| Depositing User: | Unnamed user with email elements@essex.ac.uk |
| Date Deposited: | 01 Oct 2015 09:24 |
| Last Modified: | 30 Oct 2024 19:17 |
| URI: | http://repository.essex.ac.uk/id/eprint/15104 |
Available files
Filename: 2014 Scotti---Paps Human oxygen sensing may have origins in prokaryotic EF Tu PNAS.pdf